GUAAA_SULAC
ID GUAAA_SULAC Reviewed; 188 AA.
AC Q8X239; Q4JB85;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 3.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=GMP synthase [glutamine-hydrolyzing] subunit A {ECO:0000255|HAMAP-Rule:MF_01510};
DE EC=6.3.5.2 {ECO:0000255|HAMAP-Rule:MF_01510};
DE AltName: Full=Glutamine amidotransferase {ECO:0000255|HAMAP-Rule:MF_01510};
GN Name=guaAA {ECO:0000255|HAMAP-Rule:MF_01510}; Synonyms=guaA;
GN OrderedLocusNames=Saci_0548;
OS Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS 15157 / NCIMB 11770).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfolobus.
OX NCBI_TaxID=330779;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT "The genome of Sulfolobus acidocaldarius, a model organism of the
RT Crenarchaeota.";
RL J. Bacteriol. 187:4992-4999(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-188.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RA Komorowski L., Schaefer G.;
RT "Genomic sequence coding for putative GMP synthase and quinolate
RT phosphoribosyltransferase from Sulfolobus acidocaldarius.";
RL Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of GMP from XMP. {ECO:0000255|HAMAP-
CC Rule:MF_01510}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2
CC H(+) + L-glutamate; Xref=Rhea:RHEA:11680, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:58115,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456215; EC=6.3.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01510};
CC -!- PATHWAY: Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln
CC route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_01510}.
CC -!- SUBUNIT: Heterodimer composed of a glutamine amidotransferase subunit
CC (A) and a GMP-binding subunit (B). {ECO:0000255|HAMAP-Rule:MF_01510}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC80124.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000077; AAY79944.1; -; Genomic_DNA.
DR EMBL; AJ132638; CAC80124.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_011277446.1; NC_007181.1.
DR AlphaFoldDB; Q8X239; -.
DR SMR; Q8X239; -.
DR STRING; 330779.Saci_0548; -.
DR MEROPS; C26.A31; -.
DR PRIDE; Q8X239; -.
DR EnsemblBacteria; AAY79944; AAY79944; Saci_0548.
DR GeneID; 3474610; -.
DR KEGG; sai:Saci_0548; -.
DR PATRIC; fig|330779.12.peg.533; -.
DR eggNOG; arCOG00087; Archaea.
DR HOGENOM; CLU_014340_1_4_2; -.
DR OMA; GPDMDRI; -.
DR UniPathway; UPA00189; UER00296.
DR Proteomes; UP000001018; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd01742; GATase1_GMP_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR HAMAP; MF_01510; GMP_synthase_A; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR004739; GMP_synth_GATase.
DR InterPro; IPR023686; GMP_synthase_A.
DR Pfam; PF00117; GATase; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR TIGRFAMs; TIGR00888; guaA_Nterm; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Glutamine amidotransferase; GMP biosynthesis; Ligase;
KW Nucleotide-binding; Purine biosynthesis; Reference proteome.
FT CHAIN 1..188
FT /note="GMP synthase [glutamine-hydrolyzing] subunit A"
FT /id="PRO_0000140232"
FT DOMAIN 2..188
FT /note="Glutamine amidotransferase type-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510"
FT ACT_SITE 79
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510"
FT ACT_SITE 166
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510"
FT ACT_SITE 168
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01510"
SQ SEQUENCE 188 AA; 20858 MW; 4215A86117773EB7 CRC64;
MKVAVIYFGG QYNHLIVKDL KYLGLEAVAI TPDKSVEELK EFDSVVFGGG PYSVINELDK
MGFAPDYVKS LNVPKLGICL GHQLIAKVLG GEVRKANKPE YGLTTVNIVD EDTILRGLKP
SIKAWESHND EVVRPPSGFR ILASSENAKV QAMVNNDNSI FGVQFHPEVK HTEKGIEVFK
NFIKICRK
