AMPR_PSEAE
ID AMPR_PSEAE Reviewed; 296 AA.
AC P24734;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=HTH-type transcriptional activator AmpR;
GN Name=ampR; OrderedLocusNames=PA4109;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8405939; DOI=10.1111/j.1574-6968.1993.tb06404.x;
RA Lodge J.M., Busby S.J.W., Piddock L.J.V.;
RT "Investigation of the Pseudomonas aeruginosa ampR gene and its role at the
RT chromosomal ampC beta-lactamase promoter.";
RL FEMS Microbiol. Lett. 111:315-320(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=2125210; DOI=10.1042/bj2720627;
RA Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.;
RT "Cloning, sequencing and analysis of the structural gene and regulatory
RT region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase.";
RL Biochem. J. 272:627-631(1990).
RN [4]
RP SEQUENCE REVISION TO 6.
RA Lodge J.M.;
RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This protein is a positive regulator of gene expression of
CC beta-lactamase (AmpC). {ECO:0000269|PubMed:8405939}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; X67095; CAA47470.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07496.1; -; Genomic_DNA.
DR EMBL; X54719; CAA38523.2; -; Genomic_DNA.
DR PIR; E83132; E83132.
DR PIR; S24954; S24954.
DR RefSeq; NP_252798.1; NC_002516.2.
DR RefSeq; WP_003101291.1; NZ_QZGE01000013.1.
DR PDB; 5MMH; X-ray; 2.20 A; A/B/C/D=84-296.
DR PDBsum; 5MMH; -.
DR AlphaFoldDB; P24734; -.
DR SMR; P24734; -.
DR STRING; 287.DR97_3762; -.
DR PaxDb; P24734; -.
DR PRIDE; P24734; -.
DR EnsemblBacteria; AAG07496; AAG07496; PA4109.
DR GeneID; 877983; -.
DR KEGG; pae:PA4109; -.
DR PATRIC; fig|208964.12.peg.4305; -.
DR PseudoCAP; PA4109; -.
DR HOGENOM; CLU_039613_37_0_6; -.
DR OMA; TNNNRVD; -.
DR PhylomeDB; P24734; -.
DR BioCyc; PAER208964:G1FZ6-4182-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR CollecTF; EXPREG_000009a0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1900232; P:negative regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:0050714; P:positive regulation of protein secretion; IMP:PseudoCAP.
DR GO; GO:0045862; P:positive regulation of proteolysis; IMP:PseudoCAP.
DR GO; GO:1900378; P:positive regulation of secondary metabolite biosynthetic process; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:PseudoCAP.
DR GO; GO:0006351; P:transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd08484; PBP2_LTTR_beta_lactamase; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR037420; AmpR_PBP2.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; DNA-binding; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..296
FT /note="HTH-type transcriptional activator AmpR"
FT /id="PRO_0000105589"
FT DOMAIN 6..63
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 23..42
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT CONFLICT 12
FT /note="R -> A (in Ref. 1; CAA47470 and 3; CAA38523)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="A -> G (in Ref. 1; CAA47470)"
FT /evidence="ECO:0000305"
FT STRAND 95..101
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 102..107
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 109..119
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 124..130
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 136..139
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 142..148
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 155..162
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 165..169
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 171..175
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 180..185
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 196..203
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 213..220
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 221..229
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 239..241
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 243..247
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:5MMH"
FT STRAND 263..269
FT /evidence="ECO:0007829|PDB:5MMH"
FT HELIX 276..296
FT /evidence="ECO:0007829|PDB:5MMH"
SQ SEQUENCE 296 AA; 32603 MW; 8092C376D7D91605 CRC64;
MVRPHLPLNA LRAFEASARH LSFTRAAIEL CVTQAAVSHQ VKSLEERLGV ALFKRLPRGL
MLTHEGESLL PVLCDSFDRI AGLLERFEGG HYRDVLTVGA VGTFTVGWLL PRLEDFQARH
PFIDLRLSTH NNRVDIAAEG LDYAIRFGGG AWHGTEALAL FEAPLTVLCC PEVAAQLHSP
ADLLQHTLLR SYRADEWPLW FQAAGLPAHA PLTRSIVFDT SLAMLEAARQ GVGVALAPAA
MFARQLASES IRRPFATEVS TGSYWLTRLQ SRGETSAMLA FRGWLLEMAA VEARGR
