GUAA_AQUAE
ID GUAA_AQUAE Reviewed; 510 AA.
AC O66601;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=GMP synthase [glutamine-hydrolyzing];
DE EC=6.3.5.2;
DE AltName: Full=GMP synthetase;
DE AltName: Full=Glutamine amidotransferase;
GN Name=guaA; OrderedLocusNames=aq_236;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- FUNCTION: Catalyzes the synthesis of GMP from XMP. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2
CC H(+) + L-glutamate; Xref=Rhea:RHEA:11680, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:58115,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456215; EC=6.3.5.2;
CC -!- PATHWAY: Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln
CC route): step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR EMBL; AE000657; AAC06558.1; -; Genomic_DNA.
DR PIR; E70321; E70321.
DR RefSeq; NP_213161.1; NC_000918.1.
DR RefSeq; WP_010880099.1; NC_000918.1.
DR AlphaFoldDB; O66601; -.
DR SMR; O66601; -.
DR STRING; 224324.aq_236; -.
DR MEROPS; C26.957; -.
DR PRIDE; O66601; -.
DR EnsemblBacteria; AAC06558; AAC06558; aq_236.
DR KEGG; aae:aq_236; -.
DR PATRIC; fig|224324.8.peg.193; -.
DR eggNOG; COG0518; Bacteria.
DR eggNOG; COG0519; Bacteria.
DR HOGENOM; CLU_014340_0_5_0; -.
DR InParanoid; O66601; -.
DR OMA; KRKIIGH; -.
DR OrthoDB; 504464at2; -.
DR UniPathway; UPA00189; UER00296.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003921; F:GMP synthase activity; IBA:GO_Central.
DR GO; GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006177; P:GMP biosynthetic process; IBA:GO_Central.
DR CDD; cd01742; GATase1_GMP_Synthase; 1.
DR CDD; cd01997; GMP_synthase_C; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR HAMAP; MF_00344; GMP_synthase; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR001674; GMP_synth_C.
DR InterPro; IPR004739; GMP_synth_GATase.
DR InterPro; IPR022955; GMP_synthase.
DR InterPro; IPR025777; GMPS_ATP_PPase_dom.
DR InterPro; IPR022310; NAD/GMP_synthase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF00117; GATase; 1.
DR Pfam; PF00958; GMP_synt_C; 1.
DR Pfam; PF02540; NAD_synthase; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR TIGRFAMs; TIGR00884; guaA_Cterm; 1.
DR TIGRFAMs; TIGR00888; guaA_Nterm; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
DR PROSITE; PS51553; GMPS_ATP_PPASE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Glutamine amidotransferase; GMP biosynthesis; Ligase;
KW Nucleotide-binding; Purine biosynthesis; Reference proteome.
FT CHAIN 1..510
FT /note="GMP synthase [glutamine-hydrolyzing]"
FT /id="PRO_0000140088"
FT DOMAIN 5..195
FT /note="Glutamine amidotransferase type-1"
FT DOMAIN 196..385
FT /note="GMPS ATP-PPase"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 169
FT /evidence="ECO:0000250"
FT ACT_SITE 171
FT /evidence="ECO:0000250"
FT BINDING 223..229
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 510 AA; 57858 MW; 2855029D68D1A667 CRC64;
MQRRPILVVN FGSQYVQLIA RRVRELGVYS EIVHWDTPVE EIKKKNPYGI IFSGGPASVY
AEGAPLPDKR IYELGVPILG ICYGLQVITH QLGGKVVRSE KQEYGRARLR IIKEDVIFEG
IPKESDVWMS HADKVVELPE GFEVLAVSEN SPYAVIANRE KKIYGFQFHP EVTHTVFGKE
MLANFIYGVC KAEKNWEMGD FIHEKIEEIR KTVGDAKVIA ALSGGVDSTV AAVLTHRAIG
DKLHCFFIDH GLLRYKEREE VEKNLRSLGL PLTVVDASEE FLEKLKGVED PEEKRKIIGR
TFIEVFEREA KKIEGAEFLL QGTLYPDVVE SAGIKGSAKI KTHHNVGGLP ERMNLKLLEP
FRELFKDEVR KIGKLLGVPE EILRRHPFPG PGLAIRIIGE VNKKDLEILR KADYIFIQEL
KKEGLYDRVW QAFAVLLPVK SVGVMGDVRT YEKVVALRAV ESVDGMTADW ARLPYDFLDR
VMRRIINEVE GVNRVVYDIS SKPPSTIEWE
