ID   AMPT_GEOSE              Reviewed;          30 AA.
AC   P00728;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Thermophilic aminopeptidase 1 alpha chain;
DE            EC=3.4.11.-;
DE   AltName: Full=Thermophilic aminopeptidase I alpha chain;
DE   Flags: Fragment;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924;
RX   PubMed=4521203; DOI=10.1073/pnas.70.12.3781;
RA   Stoll E., Ericsson L.H., Zuber H.;
RT   "The function of the two subunits of thermophilic aminopeptidase I.";
RL   Proc. Natl. Acad. Sci. U.S.A. 70:3781-3784(1973).
CC   -!- FUNCTION: Metalloenzyme of broad specificity, releasing all N-terminal
CC       amino acids.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Binds 2 divalent metal cations per subunit. {ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Highly thermostable.;
CC   -!- SUBUNIT: 12 chains of two different but homologous types, alpha and
CC       beta, which can combine in various ratios.
CC   -!- SIMILARITY: Belongs to the peptidase M42 family. {ECO:0000305}.
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DR   PIR; A00908; AIBSAF.
DR   AlphaFoldDB; P00728; -.
DR   SMR; P00728; -.
DR   PRIDE; P00728; -.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Aminopeptidase; Direct protein sequencing; Hydrolase; Metalloprotease;
KW   Protease.
FT   CHAIN           1..>30
FT                   /note="Thermophilic aminopeptidase 1 alpha chain"
FT                   /id="PRO_0000071652"
FT   NON_TER         30
SQ   SEQUENCE   30 AA;  3274 MW;  D712C9C23E618142 CRC64;
     AKLDETLTML KALTDAKGVP GNEREARDVM