ID   AMP_BRELA               Reviewed;          11 AA.
AC   B3EWC5;
DT   03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Antimicrobial protein BL-A60 {ECO:0000303|PubMed:22244810};
OS   Brevibacillus laterosporus (Bacillus laterosporus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX   NCBI_TaxID=1465;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=A60 {ECO:0000269|PubMed:22244810};
RX   PubMed=22244810; DOI=10.1016/j.peptides.2012.01.001;
RA   Zhao J., Guo L., Zeng H., Yang X., Yuan J., Shi H., Xiong Y., Chen M.,
RA   Han L., Qiu D.;
RT   "Purification and characterization of a novel antimicrobial peptide from
RT   Brevibacillus laterosporus strain A60.";
RL   Peptides 33:206-211(2012).
CC   -!- FUNCTION: Has antibacterial activity against P.solanaceum (MIC=10 uM),
CC       X.vesicolor (MIC=100 uM) and B.subtilis (MIC=5 uM). Has antifungal
CC       activity against P.capsici (MIC=15 uM), B.cinerea (MIC=25 uM),
CC       V.dahliae (MIC=200 uM) and F.omysporum (MIC=50 uM). In P.capsici,
CC       inhibits mycelial growth (EC(50)=7.89 ug/ml), formation of sporangia
CC       (EC(50)=0.6 ug/ml) and cytospore germination (EC(50)=21.96 ug/ml).
CC       {ECO:0000269|PubMed:22244810}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Active between pH 3 and 11. {ECO:0000269|PubMed:22244810};
CC       Temperature dependence:
CC         Retains >80% activity after 15 min at 100 degrees Celsius.
CC         {ECO:0000269|PubMed:22244810};
CC   -!- PTM: Contains a choline moiety at the N-terminus and a tenuazonic acid
CC       moiety at the C-terminus. {ECO:0000269|PubMed:22244810}.
CC   -!- MASS SPECTROMETRY: Mass=1602.0469; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22244810};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide.
FT   PEPTIDE         1..11
FT                   /note="Antimicrobial protein BL-A60"
FT                   /evidence="ECO:0000269|PubMed:22244810"
FT                   /id="PRO_0000421853"
SQ   SEQUENCE   11 AA;  1320 MW;  527D644ED2C322C7 CRC64;
     LYKLVKVVLN M