ID   AMP_ONYPE               Reviewed;         233 AA.
AC   Q7M1T6; P81957;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2004, sequence version 3.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Antigenic membrane protein;
DE   AltName: Full=Immunodominant membrane protein;
DE   Flags: Precursor;
GN   Name=amp; OrderedLocusNames=PAM_122;
OS   Onion yellows phytoplasma (strain OY-M).
OC   Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae;
OC   Candidatus Phytoplasma; Candidatus Phytoplasma asteris.
OX   NCBI_TaxID=262768;
RN   [1] {ECO:0000312|EMBL:BAD07009.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14702406; DOI=10.1099/mic.0.26521-0;
RA   Kakizawa S., Oshima K., Nishigawa H., Jung H.-Y., Wei W., Suzuki S.,
RA   Tanaka M., Miyata S., Ugaki M., Namba S.;
RT   "Secretion of immunodominant membrane protein from Onion yellows
RT   phytoplasma through the Sec protein-translocation system in Escherichia
RT   coli.";
RL   Microbiology 150:135-142(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OY-M;
RX   PubMed=14661021; DOI=10.1038/ng1277;
RA   Oshima K., Kakizawa S., Nishigawa H., Jung H.-Y., Wei W., Suzuki S.,
RA   Arashida R., Nakata D., Miyata S., Ugaki M., Namba S.;
RT   "Reductive evolution suggested from the complete genome sequence of a
RT   plant-pathogenic phytoplasma.";
RL   Nat. Genet. 36:27-29(2004).
RN   [3] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 33-73.
RC   STRAIN=OY-W {ECO:0000305};
RA   Zhon B., Tanaka M., Matsuda I.;
RT   "Analysis of phytoplasmal proteins isolated by two dimensional gel
RT   electrophoresis.";
RL   Submitted (JUL-1999) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AB110271; BAD07009.1; -; Genomic_DNA.
DR   EMBL; AP006628; BAD04207.1; -; Genomic_DNA.
DR   PIR; A58995; A58995.
DR   AlphaFoldDB; Q7M1T6; -.
DR   SMR; Q7M1T6; -.
DR   EnsemblBacteria; BAD04207; BAD04207; PAM_122.
DR   KEGG; poy:PAM_122; -.
DR   HOGENOM; CLU_1188981_0_0_14; -.
DR   OMA; TLECKDA; -.
DR   BioCyc; OYEL262768:G1G26-154-MON; -.
DR   Proteomes; UP000002523; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR029216; Phyto-Amp.
DR   Pfam; PF15438; Phyto-Amp; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000269|Ref.3"
FT   CHAIN           33..233
FT                   /note="Antigenic membrane protein"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000020723"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        6
FT                   /note="N -> T (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        39
FT                   /note="N -> S (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        70
FT                   /note="S -> T (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        107..109
FT                   /note="ILN -> VLS (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        124
FT                   /note="E -> D (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        214
FT                   /note="V -> F (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        231
FT                   /note="K -> N (in Ref. 1; BAD07009)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   233 AA;  24743 MW;  715E9D3AFB3103E7 CRC64;
     MQNQKNQKSL VAKVLVLFAA VALMFVGVQV FADDKLDLNT LECKDALELT AADAADAEKV
     VKQWKVQNTS LNAKVTKDSV KVAVADNKVT VTPADGDAGK ALSGSKILNL VGVCELNKLT
     LGTEKKLTLT VKDGKVDAEA GLKALKEAGA KVPATVNKDD VTFTVGKDDN ANKVTVKAVD
     GKTTVSGQVV FEFTVAKTPW YKTVWFLTLV AVVVVAAVAG GVFFFVKKNK KNK