GUAA_NEIG2
ID GUAA_NEIG2 Reviewed; 521 AA.
AC B4RJH7;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=GMP synthase [glutamine-hydrolyzing] {ECO:0000255|HAMAP-Rule:MF_00344};
DE EC=6.3.5.2 {ECO:0000255|HAMAP-Rule:MF_00344};
DE AltName: Full=GMP synthetase {ECO:0000255|HAMAP-Rule:MF_00344};
DE AltName: Full=Glutamine amidotransferase {ECO:0000255|HAMAP-Rule:MF_00344};
GN Name=guaA {ECO:0000255|HAMAP-Rule:MF_00344}; OrderedLocusNames=NGK_2643;
OS Neisseria gonorrhoeae (strain NCCP11945).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=521006;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCCP11945;
RX PubMed=18586945; DOI=10.1128/jb.00566-08;
RA Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.;
RT "Complete genome sequence of Neisseria gonorrhoeae NCCP11945.";
RL J. Bacteriol. 190:6035-6036(2008).
CC -!- FUNCTION: Catalyzes the synthesis of GMP from XMP. {ECO:0000255|HAMAP-
CC Rule:MF_00344}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2
CC H(+) + L-glutamate; Xref=Rhea:RHEA:11680, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:58115,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456215; EC=6.3.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00344};
CC -!- PATHWAY: Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln
CC route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00344}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00344}.
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DR EMBL; CP001050; ACF31242.1; -; Genomic_DNA.
DR RefSeq; WP_003702177.1; NC_011035.1.
DR PDB; 5TW7; X-ray; 2.35 A; A/B/C/D/E/F=1-521.
DR PDBsum; 5TW7; -.
DR AlphaFoldDB; B4RJH7; -.
DR SMR; B4RJH7; -.
DR MEROPS; C26.957; -.
DR EnsemblBacteria; ACF31242; ACF31242; NGK_2643.
DR KEGG; ngk:NGK_2643; -.
DR HOGENOM; CLU_014340_0_5_4; -.
DR OMA; KRKIIGH; -.
DR OrthoDB; 504464at2; -.
DR UniPathway; UPA00189; UER00296.
DR Proteomes; UP000002564; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd01742; GATase1_GMP_Synthase; 1.
DR CDD; cd01997; GMP_synthase_C; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR HAMAP; MF_00344; GMP_synthase; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR001674; GMP_synth_C.
DR InterPro; IPR004739; GMP_synth_GATase.
DR InterPro; IPR022955; GMP_synthase.
DR InterPro; IPR025777; GMPS_ATP_PPase_dom.
DR InterPro; IPR022310; NAD/GMP_synthase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF00117; GATase; 1.
DR Pfam; PF00958; GMP_synt_C; 1.
DR Pfam; PF02540; NAD_synthase; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR TIGRFAMs; TIGR00884; guaA_Cterm; 1.
DR TIGRFAMs; TIGR00888; guaA_Nterm; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
DR PROSITE; PS51553; GMPS_ATP_PPASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Glutamine amidotransferase; GMP biosynthesis;
KW Ligase; Nucleotide-binding; Purine biosynthesis.
FT CHAIN 1..521
FT /note="GMP synthase [glutamine-hydrolyzing]"
FT /id="PRO_1000120341"
FT DOMAIN 5..197
FT /note="Glutamine amidotransferase type-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT DOMAIN 198..390
FT /note="GMPS ATP-PPase"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT ACT_SITE 81
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT ACT_SITE 171
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT ACT_SITE 173
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT BINDING 225..231
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00344"
FT STRAND 5..10
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 16..25
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 39..45
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 48..52
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 68..72
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 77..80
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 82..90
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 94..96
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 103..110
FT /evidence="ECO:0007829|PDB:5TW7"
FT TURN 114..118
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 126..132
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 134..139
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 145..150
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 153..160
FT /evidence="ECO:0007829|PDB:5TW7"
FT TURN 161..164
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 165..170
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 180..189
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 200..215
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 228..241
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 242..244
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 245..251
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 259..271
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 274..279
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 281..288
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 294..315
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 321..323
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 328..331
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 361..363
FT /evidence="ECO:0007829|PDB:5TW7"
FT TURN 365..368
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 371..381
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 385..388
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 397..400
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 408..427
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 436..439
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 441..454
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 461..473
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 479..481
FT /evidence="ECO:0007829|PDB:5TW7"
FT HELIX 486..499
FT /evidence="ECO:0007829|PDB:5TW7"
FT STRAND 503..509
FT /evidence="ECO:0007829|PDB:5TW7"
SQ SEQUENCE 521 AA; 57656 MW; 208D32100AA27D2D CRC64;
MTQDKILILD FGSQVTRLIA RRVREAHVYC ELHSFDMPLD EIKAFNPKGI ILSGGPNSVY
ESDYQADTGI FDLGIPVLGI CYGMQFMAHH LGGEVQPGNQ REFGYAQVKT IDSGLTRGIQ
DDAPNTLDVW MSHGDKVSKL PDGFAVIGDT PSCPIAMMEN TEKQFYGIQF HPEVTHTKQG
RALLNRFVLD ICGAQPGWTM PNYIEEAVAK IREQVGSDEV ILGLSGGVDS SVAAALIHRA
IGDQLTCVFV DHGLLRLNEG KMVMDMFARN LGVKVIHVDA EGQFMAKLAG VTDPEKKRKI
IGAEFIEVFD AEEKKLTNAK WLAQGTIYPD VIESAGAKTK KAHAIKSHHN VGGLPENMKL
KLLEPLRDLF KDEVRELGVA LGLPREMVYR HPFPGPGLGV RILGEVKKEY ADLLRQADDI
FIQELRNTTD ENGTSWYDLT SQAFAVFLPV KSVGVMGDGR TYDYVVALRA VITSDFMTAH
WAELPYSLLG RVSNRIINEV KGINRVVYDV SGKPPATIEW E
