ID   AMSA_ERWAM              Reviewed;         726 AA.
AC   Q46631;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Putative tyrosine-protein kinase AmsA;
DE            EC=2.7.10.-;
DE   AltName: Full=Amylovoran biosynthesis membrane-associated protein AmsA;
GN   Name=amsA;
OS   Erwinia amylovora (Fire blight bacteria).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=552;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EA1/79;
RX   PubMed=7596293; DOI=10.1111/j.1365-2958.1995.tb02361.x;
RA   Bugert P., Geider K.;
RT   "Molecular analysis of the ams operon required for exopolysaccharide
RT   synthesis of Erwinia amylovora.";
RL   Mol. Microbiol. 15:917-933(1995).
CC   -!- FUNCTION: Involved in the biosynthesis of amylovoran which functions as
CC       a virulence factor.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC         [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC         COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC         ChEBI:CHEBI:82620, ChEBI:CHEBI:456216;
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the etk/wzc family. {ECO:0000305}.
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DR   EMBL; X77921; CAA54882.1; -; Genomic_DNA.
DR   PIR; S61894; S52141.
DR   RefSeq; WP_004158326.1; NZ_RQKG01000006.1.
DR   AlphaFoldDB; Q46631; -.
DR   SMR; Q46631; -.
DR   PRIDE; Q46631; -.
DR   GeneID; 8912406; -.
DR   OMA; QQIYIQL; -.
DR   UniPathway; UPA00631; -.
DR   PHI-base; PHI:2471; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR025669; AAA_dom.
DR   InterPro; IPR005702; EPS_synthesis.
DR   InterPro; IPR032807; GNVR.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF13614; AAA_31; 1.
DR   Pfam; PF13807; GNVR; 1.
DR   Pfam; PF02706; Wzz; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01007; eps_fam; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane;
KW   Exopolysaccharide synthesis; Kinase; Membrane; Nucleotide-binding;
KW   Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase;
KW   Virulence.
FT   CHAIN           1..726
FT                   /note="Putative tyrosine-protein kinase AmsA"
FT                   /id="PRO_0000212357"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   726 AA;  80400 MW;  1D97E7A8395EF348 CRC64;
     MKSKIEEPSA NGGEAIRFEL AHLLGQLLDH RWMIVAVSVL FTLMGTLYSL FATPIYSADA
     MVQVEQKNAN TVLNDISQMM PNAQPASAAE IEIITSRMVL GKTVADLGLD VLVQQDHFPL
     IGAGLSRIIG QKAQQIAVSR LKVPTLWDKR ELSVEVDGPD SYTVSKDGNE LFKGKVGQFE
     QHGDVTMLVN SIEADAGTRF TVSKLNNLQA IKMISNNLVV ADMGKDTGVL GLTYSGEDPV
     QISRVLDQVI NNYLYQNIAR KSEEAEKSIQ FLAQQLPDVR AKLDQAEDKL NVFRRKHDSV
     DMSLEAKSAL DSSVSIQTQL NALTFREAEV SQLFKKDHPT YRALLEKRQT LDEQQKQLNG
     KISQMPQTQQ EIVRLTRDVQ AGQEIYMQLL NRQQELNISK ASTVGDVRII DHAETAAKPV
     APKSILIVAG SLILGLVVSV GLVLMKALFH HGIDNPEQLE ELGLNVYASV PLSEWQRKKD
     QETLLKRKLD ARTDPHNRLL ALGNPTDLSI EAIRSLRTSL HFAMMDAQNN ILMITGASPG
     IGKTFVCANL ATLVAKTGEK VLFIDGDMRR GYTHELLGAE SKTGLSDILS GKLPFNTDLV
     QRGDYGFDFI ARGQVPPNPS ELLMNSRMKE LVHWASQNYD LVLIDTPPIL AVTDASIIGK
     LAGTSLMVAR FETNTVKEVE ISYKRFIQNG IDIKGIILNA VVRKSANNYG YGYDYYDYSY
     QQGEKS