AMT12_ARATH
ID AMT12_ARATH Reviewed; 514 AA.
AC Q9ZPJ8; Q9SQH8;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Ammonium transporter 1 member 2;
DE Short=AtAMT1;2;
GN Name=AMT1-2; OrderedLocusNames=At1g64780; ORFNames=F13O11.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. C24; TISSUE=Seed;
RX PubMed=10330477; DOI=10.2307/3870826;
RA Gazzarrini S., Lejay L., Gojon A., Ninnemann O., Frommer W.B.,
RA von Wiren N.;
RT "Three functional transporters for constitutive, diurnally regulated, and
RT starvation-induced uptake of ammonium into Arabidopsis roots.";
RL Plant Cell 11:937-948(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24; TISSUE=Root;
RA Shelden M.C., Howitt S.M., Udvardi M.K.;
RT "Arabidopsis thaliana AtAMT1;2 from N-deprived roots.";
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-472, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: Ammonium transporter probably involved in ammonium uptake
CC from the soil. {ECO:0000269|PubMed:10330477}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: High expression in root.
CC {ECO:0000269|PubMed:10330477}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; AF083036; AAD54639.1; -; mRNA.
DR EMBL; AF110771; AAD17001.1; -; mRNA.
DR EMBL; AC006193; AAD38253.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34288.1; -; Genomic_DNA.
DR EMBL; AY062571; AAL32649.1; -; mRNA.
DR EMBL; AY093374; AAM13373.1; -; mRNA.
DR PIR; A96671; A96671.
DR RefSeq; NP_176658.1; NM_105152.3.
DR AlphaFoldDB; Q9ZPJ8; -.
DR SMR; Q9ZPJ8; -.
DR BioGRID; 28007; 8.
DR IntAct; Q9ZPJ8; 1.
DR STRING; 3702.AT1G64780.1; -.
DR iPTMnet; Q9ZPJ8; -.
DR PaxDb; Q9ZPJ8; -.
DR PRIDE; Q9ZPJ8; -.
DR ProteomicsDB; 240319; -.
DR EnsemblPlants; AT1G64780.1; AT1G64780.1; AT1G64780.
DR GeneID; 842786; -.
DR Gramene; AT1G64780.1; AT1G64780.1; AT1G64780.
DR KEGG; ath:AT1G64780; -.
DR Araport; AT1G64780; -.
DR TAIR; locus:2010791; AT1G64780.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_1_1; -.
DR InParanoid; Q9ZPJ8; -.
DR OMA; MACWNSN; -.
DR OrthoDB; 910733at2759; -.
DR PhylomeDB; Q9ZPJ8; -.
DR PRO; PR:Q9ZPJ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZPJ8; baseline and differential.
DR Genevisible; Q9ZPJ8; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR GO; GO:0015843; P:methylammonium transport; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Ammonium transporter 1 member 2"
FT /id="PRO_0000139744"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 472
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19245862"
FT CONFLICT 198
FT /note="R -> G (in Ref. 1; AAD54639)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="A -> S (in Ref. 1; AAD54639)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="V -> I (in Ref. 1; AAD54639)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 55014 MW; 86C3C61F044A0CC6 CRC64;
MDTATTTCSA VDLSALLSSS SNSTSSLAAA TFLCSQISNI SNKLSDTTYA VDNTYLLFSA
YLVFAMQLGF AMLCAGSVRA KNTMNIMLTN VLDAAAGAIS YYLFGFAFAF GTPSNGFIGR
HHSFFALSSY PERPGSDFSF FLYQWAFAIA AAGITSGSIA ERTQFVAYLI YSTFLTGFVY
PTVSHWFWSS DGWASASRSD NNLLFGSGAI DFAGSGVVHM VGGIAGLCGA LVEGPRIGRF
DRSGRSVALR GHSASLVVLG TFLLWFGWYG FNPGSFLTIL KGYDKSRPYY GQWSAVGRTA
VTTTLSGCTA ALTTLFSKRL LAGHWNVIDV CNGLLGGFAA ITSGCAVVEP WAAIVCGFVA
SWVLIGFNLL AKKLKYDDPL EAAQLHGGCG AWGLIFTGLF ARKEYVNEIY SGDRPYGLFM
GGGGKLLAAQ IVQIIVIVGW VTVTMGPLFY GLHKMNLLRI SAEDEMAGMD MTRHGGFAYA
YNDEDDVSTK PWGHFAGRVE PTSRSSTPTP TLTV
