ID   AMT12_SOLLC             Reviewed;         514 AA.
AC   O04161;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Ammonium transporter 1 member 2;
DE   AltName: Full=LeAMT1;2;
GN   Name=AMT1-2;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RC   TISSUE=Root hair;
RX   PubMed=10743657; DOI=10.1046/j.1365-313x.2000.00665.x;
RA   von Wiren N., Lauter F.-R., Ninnemann O., Gillissen B., Walch-Liu P.,
RA   Engels C., Jost W., Frommer W.B.;
RT   "Differential regulation of three functional ammonium transporter genes by
RT   nitrogen in root hairs and by light in leaves of tomato.";
RL   Plant J. 21:167-175(2000).
RN   [2]
RP   INDUCTION.
RX   PubMed=12111224; DOI=10.1007/s00425-002-0773-x;
RA   Becker D., Stanke R., Fendrik I., Frommer W.B., Vanderleyden J.,
RA   Kaiser W.M., Hedrich R.;
RT   "Expression of the NH(+)(4)-transporter gene LEAMT1;2 is induced in tomato
RT   roots upon association with N(2)-fixing bacteria.";
RL   Planta 215:424-429(2002).
CC   -!- FUNCTION: Ammonium transporter probably involved in ammonium uptake
CC       from the soil. {ECO:0000269|PubMed:10743657}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Root hairs and leaves.
CC   -!- INDUCTION: By ammonium supply or colonization by nitrogen-fixing
CC       bacteria in roots. Down-regulated by glutamine supply in roots. Highest
CC       expression after onset of light. {ECO:0000269|PubMed:10743657,
CC       ECO:0000269|PubMed:12111224}.
CC   -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X95098; CAA64475.1; -; mRNA.
DR   PIR; T06585; T06585.
DR   AlphaFoldDB; O04161; -.
DR   SMR; O04161; -.
DR   STRING; 4081.Solyc04g050440.2.1; -.
DR   PaxDb; O04161; -.
DR   PRIDE; O04161; -.
DR   eggNOG; KOG0682; Eukaryota.
DR   InParanoid; O04161; -.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; O04161; baseline and differential.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR   GO; GO:0072488; P:ammonium transmembrane transport; IGI:UniProtKB.
DR   Gene3D; 1.10.3430.10; -; 1.
DR   InterPro; IPR029020; Ammonium/urea_transptr.
DR   InterPro; IPR001905; Ammonium_transpt.
DR   InterPro; IPR018047; Ammonium_transpt_CS.
DR   InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR   Pfam; PF00909; Ammonium_transp; 1.
DR   TIGRFAMs; TIGR00836; amt; 1.
DR   PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE   2: Evidence at transcript level;
KW   Ammonia transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..514
FT                   /note="Ammonium transporter 1 member 2"
FT                   /id="PRO_0000139749"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        428..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   514 AA;  55544 MW;  9987FE9FE17173C9 CRC64;
     MASAMTCSAA ELFPHLGSSA NATAAAEFIC SRFSAVSEYL TNTTYAVDTT YLLFSAYLVF
     AMQLGFAMLC AGSVRAKNTM NIMLTNVLDA AAGGFSYYLF GFAFAFGAPS NGFIGKHFFG
     LKEFPSQAFD YSYFLYQWAF AIAAAGITSG SIAERTQFVA YLIYSSFLTG FVYPIVSHWF
     WSGDGWASAS KTDGNLLLRF GVIDFAGSGV VHMVGGIAGL WGAFIEGPRI GRFDRSGRSV
     ALRGHSASLV VLGTFLLWFG WYGFNPGSFL TILKSYDHTI RGTYYGQWSA IGRTAVTTTL
     AGCTAALTTL FCKRLLVAHW NVVDVCNGLL GGFAAITSGC AVVEPWAAIV CGFIAAWVLI
     GFNALAAKLK YDDPLEAAQL HGGCGSWGII FTGLFAKKEY VNEVYPGFPN RPYGLFMGGG
     GKLLGAQVIQ VVVIIGWVSV TMGPLFYLLH KFKLLRISRD DETAGMDLTR HGGFAYIYHD
     EDEGSSMPGF KMTRVEPTNT STPDHQNRSV NVVV