AMT14_ARATH
ID AMT14_ARATH Reviewed; 504 AA.
AC Q9SVT8; Q4PSH7;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Ammonium transporter 1 member 4;
DE Short=AtAMT1;4;
GN Name=AMT1-4; OrderedLocusNames=At4g28700; ORFNames=F16A16.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19073648; DOI=10.1093/pcp/pcn186;
RA Yuan L., Graff L., Loque D., Kojima S., Tsuchiya Y.N., Takahashi H.,
RA von Wiren N.;
RT "AtAMT1;4, a pollen-specific high-affinity ammonium transporter of the
RT plasma membrane in Arabidopsis.";
RL Plant Cell Physiol. 50:13-25(2009).
CC -!- FUNCTION: High affinity ammonium transporter in the plasma membrane.
CC {ECO:0000269|PubMed:19073648}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=17 uM for ammonium chloride {ECO:0000269|PubMed:19073648};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19073648};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19073648}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen grains and tubes.
CC {ECO:0000269|PubMed:19073648}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; AL035353; CAA22982.1; -; Genomic_DNA.
DR EMBL; AL161573; CAB81458.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85527.1; -; Genomic_DNA.
DR EMBL; DQ056659; AAY78806.1; -; mRNA.
DR PIR; T04529; T04529.
DR RefSeq; NP_194599.1; NM_119012.2.
DR AlphaFoldDB; Q9SVT8; -.
DR SMR; Q9SVT8; -.
DR BioGRID; 14275; 8.
DR IntAct; Q9SVT8; 7.
DR STRING; 3702.AT4G28700.1; -.
DR TCDB; 1.A.11.2.6; the ammonium transporter channel (amt) family.
DR PaxDb; Q9SVT8; -.
DR PRIDE; Q9SVT8; -.
DR ProteomicsDB; 244467; -.
DR EnsemblPlants; AT4G28700.1; AT4G28700.1; AT4G28700.
DR GeneID; 828988; -.
DR Gramene; AT4G28700.1; AT4G28700.1; AT4G28700.
DR KEGG; ath:AT4G28700; -.
DR Araport; AT4G28700; -.
DR TAIR; locus:2117758; AT4G28700.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_1_1; -.
DR InParanoid; Q9SVT8; -.
DR OMA; HFAFIRT; -.
DR OrthoDB; 910733at2759; -.
DR PhylomeDB; Q9SVT8; -.
DR SABIO-RK; Q9SVT8; -.
DR PRO; PR:Q9SVT8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SVT8; baseline and differential.
DR Genevisible; Q9SVT8; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015398; F:high-affinity secondary active ammonium transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IDA:TAIR.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..504
FT /note="Ammonium transporter 1 member 4"
FT /id="PRO_0000139746"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 471
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P54144"
SQ SEQUENCE 504 AA; 53658 MW; 089B12A9EE06FCDA CRC64;
MASALSCSAS DLIPLLSGGA NATAAAAAAE YICGRFDTVA GKFTDAAYAI DNTYLLFSAY
LVFAMQLGFA MLCAGSVRAK NTMNIMLTNV IDAAAGGLFY YLFGFAFAFG SPSNGFIGKH
FFGMYDFPQP TFDYPYFLYQ WTFAIAAAGI TSGSIAERTQ FVAYLIYSSF LTGLVYPIVS
HWFWSSDGWA SPARSENLLF QSGVIDFAGS GVVHMVGGIA GLWGALIEGP RIGRFGVGGK
PVTLRGHSAT LVVLGTFLLW FGWYGFNPGS FATIFKAYGE TPGSSFYGQW SAVGRTAVTT
TLAGCTAALT TLFGKRLIDG YWNVTDVCNG LLGGFAAITS GCSVVEPWAA LVCGFVAAWV
LMGCNRLAEK LQFDDPLEAA QLHGGCGAWG IIFTGLFAEK RYIAEIFGGD PNRPFGLLMG
GGGRLLAAHV VQILVITGWV SVTMGTLFFI LHKLKLLRIP AEDEIAGVDP TSHGGLAYMY
TEDEIRNGIM VRRVGGDNDP NVGV
