AMT1_DICDI
ID AMT1_DICDI Reviewed; 463 AA.
AC Q9BLG4; Q54ZJ7;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ammonium transporter 1;
GN Name=amtA; ORFNames=DDB_G0277503;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX2;
RA Chen Y., Huang C.-H.;
RT "Cloning and characterization of three ammonium transporter genes in AX2
RT strain of Dictyostelium discoideum.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AX4;
RX PubMed=17951519; DOI=10.1128/ec.00204-07;
RA Yoshino R., Morio T., Yamada Y., Kuwayama H., Sameshima M., Tanaka Y.,
RA Sesaki H., Iijima M.;
RT "Regulation of ammonia homeostasis by the ammonium transporter AmtA in
RT Dictyostelium discoideum.";
RL Eukaryot. Cell 6:2419-2428(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=14686953; DOI=10.1111/j.1432-0436.2003.07109002.x;
RA Follstaedt S.C., Kirsten J.H., Singleton C.K.;
RT "Temporal and spatial expression of ammonium transporter genes during
RT growth and development of Dictyostelium discoideum.";
RL Differentiation 71:557-566(2003).
RN [6]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=16835443; DOI=10.1128/ec.00058-06;
RA Singleton C.K., Kirsten J.H., Dinsmore C.J.;
RT "Function of ammonium transporter A in the initiation of culmination of
RT development in Dictyostelium discoideum.";
RL Eukaryot. Cell 5:991-996(2006).
RN [7]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=19108721; DOI=10.1186/1471-2121-9-71;
RA Kirsten J.H., Xiong Y., Davis C.T., Singleton C.K.;
RT "Subcellular localization of ammonium transporters in Dictyostelium
RT discoideum.";
RL BMC Cell Biol. 9:71-71(2008).
CC -!- FUNCTION: Ammonium transporter that mediates the excretion of ammonium.
CC Controls ammonium homeostasis during growth and development. Ammonium
CC has been shown to function as a morphogen at multiple steps during the
CC development. {ECO:0000269|PubMed:14686953, ECO:0000269|PubMed:16835443,
CC ECO:0000269|PubMed:17951519}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19108721};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19108721}. Endosome
CC membrane {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Lysosome membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}.
CC -!- DEVELOPMENTAL STAGE: Predominantly found in the prestalk of the slug.
CC {ECO:0000269|PubMed:14686953}.
CC -!- DISRUPTION PHENOTYPE: Shortens transitional period (when fingers and
CC migrating slugs assess their environment to determine the
CC appropriateness of that environment for culmination and fruiting body
CC formation) of 1 to 2 hours and rarely forms slugs under standard
CC conditions of development. Gives a slightly enhanced growth rate in
CC axenic cultures. {ECO:0000269|PubMed:16835443}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; AF510716; AAP47145.1; -; mRNA.
DR EMBL; AB049067; BAB39709.1; -; Genomic_DNA.
DR EMBL; AAFI02000020; EAL68714.1; -; Genomic_DNA.
DR RefSeq; XP_642645.1; XM_637553.1.
DR AlphaFoldDB; Q9BLG4; -.
DR SMR; Q9BLG4; -.
DR STRING; 44689.DDB0185017; -.
DR TCDB; 1.A.11.1.7; the ammonium transporter channel (amt) family.
DR PaxDb; Q9BLG4; -.
DR EnsemblProtists; EAL68714; EAL68714; DDB_G0277503.
DR GeneID; 8621061; -.
DR KEGG; ddi:DDB_G0277503; -.
DR dictyBase; DDB_G0277503; amtA.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_0_1; -.
DR InParanoid; Q9BLG4; -.
DR OMA; PHNVPFI; -.
DR PhylomeDB; Q9BLG4; -.
DR PRO; PR:Q9BLG4; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0000421; C:autophagosome membrane; IDA:dictyBase.
DR GO; GO:0036020; C:endolysosome membrane; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0005774; C:vacuolar membrane; IDA:dictyBase.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:dictyBase.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:dictyBase.
DR GO; GO:0019954; P:asexual reproduction; IMP:dictyBase.
DR GO; GO:0031159; P:positive regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0046903; P:secretion; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Cytoplasmic vesicle; Endosome; Lysosome;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..463
FT /note="Ammonium transporter 1"
FT /id="PRO_0000365573"
FT TOPO_DOM 1..39
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..127
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..194
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..265
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 315..316
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..392
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..463
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
SQ SEQUENCE 463 AA; 49051 MW; 5EAF9DD8A69EAD80 CRC64;
MVAGEIIKGV AAEITNGSSS SVVQKYLDCA NQVAPDPGNT TWVLLSTILV LGMMPALAFF
EAGLLRSKNT LSIITQIMSG IVVLTVMWQA FGYSLTFGPD QKGIIGNLDH AFLINVSYDD
CSPNAPNIPA AAYAFFMMMF ANITPLLMTG AFAERVKFKA FIALTVAWEI IVFYPVAHWI
WGGGWLHKYF GVLDFAGGIV IHTSAGVSAL VIALYVGRRK DFEKYGGEFP PSNLPLATIG
AALLWMGWFG FNAGSALAAG NIATSAVAST QIGGSFSAIV WIILSAAKGK PNTVSVINGV
IAGLAGITPA SGYINSQYSI GLGICLGLAS YYSVVLLKHK LHIDDALDVS SVHGLTGIIG
SLAIGFCAEL SVNPNGANGA FYGNPKLIGT QLLGVVSVAV WAAAWTWVLL KIIDATIGVK
IDESEEELGL DLVEHGEFAY HNISLQGNEN HYSSVINSHD FFK
