ID   GUB_BACAM               Reviewed;         239 AA.
AC   P07980;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Beta-glucanase;
DE            EC=3.2.1.73;
DE   AltName: Full=1,3-1,4-beta-D-glucan 4-glucanohydrolase;
DE   AltName: Full=Endo-beta-1,3-1,4 glucanase;
DE   AltName: Full=Lichenase;
DE   Flags: Precursor;
GN   Name=bglA;
OS   Bacillus amyloliquefaciens (Bacillus velezensis).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BE 20/78;
RX   PubMed=3106158; DOI=10.1016/0378-1119(86)90278-7;
RA   Hofemeister J., Kurtz A., Borriss R., Knowles J.;
RT   "The beta-glucanase gene from Bacillus amyloliquefaciens shows extensive
RT   homology with that of Bacillus subtilis.";
RL   Gene 49:177-187(1986).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-
CC         glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73;
CC   -!- MISCELLANEOUS: Beta-glucanases of Bacillus have a substrate range
CC       similar to lichenase of germinating barley.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. {ECO:0000305}.
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DR   EMBL; M15674; AAA87323.1; -; Genomic_DNA.
DR   PIR; A29091; A29091.
DR   AlphaFoldDB; P07980; -.
DR   SMR; P07980; -.
DR   STRING; 692420.BAMF_3732; -.
DR   CAZy; GH16; Glycoside Hydrolase Family 16.
DR   eggNOG; COG2273; Bacteria.
DR   BRENDA; 3.2.1.73; 630.
DR   GO; GO:0042972; F:licheninase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR044791; Beta-glucanase/XTH.
DR   InterPro; IPR008264; Beta_glucanase.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR000757; GH16.
DR   InterPro; IPR008263; GH16_AS.
DR   PANTHER; PTHR31062; PTHR31062; 1.
DR   Pfam; PF00722; Glyco_hydro_16; 1.
DR   PRINTS; PR00737; GLHYDRLASE16.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS01034; GH16_1; 1.
DR   PROSITE; PS51762; GH16_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..25
FT   CHAIN           26..239
FT                   /note="Beta-glucanase"
FT                   /id="PRO_0000011786"
FT   DOMAIN          26..239
FT                   /note="GH16"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01098"
FT   ACT_SITE        134
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10064"
FT   DISULFID        57..86
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   239 AA;  26928 MW;  A76A64268A7AAA0B CRC64;
     MKRVLLILVT GLFMSLCGIT SSVSAQTGGS FFEPFNSYNS GLWQKADGYS NGDMFNCTWR
     ANNVSMTSLG EMRLALTSPS YNKFDCGENR SVQTYGYGLY EVRMKPAKNT GIVSSFFTYT
     GPTEGTPWDE IDIEFLGKDT TKVQFNYYTN GAGNHEKFAD LGFDAANAYH TYAFDWQPNS
     IKWYVDGQLK HTATTQIPAA PGKIMMNLWN GTGVDDWLGS YNGVNPIYAH YDWMRYRKK