GUB_FIBSS
ID GUB_FIBSS Reviewed; 349 AA.
AC P17989; C9RPK9; D9S550;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Beta-glucanase;
DE EC=3.2.1.73;
DE AltName: Full=1,3-1,4-beta-D-glucan 4-glucanohydrolase;
DE AltName: Full=Endo-beta-1,3-1,4 glucanase;
DE AltName: Full=Lichenase;
DE AltName: Full=Mixed linkage beta-glucanase;
DE Flags: Precursor;
GN OrderedLocusNames=Fisuc_2961, FSU_0226;
OS Fibrobacter succinogenes (strain ATCC 19169 / S85).
OC Bacteria; Fibrobacteres; Fibrobacterales; Fibrobacteraceae; Fibrobacter.
OX NCBI_TaxID=59374;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 28-57.
RX PubMed=2193918; DOI=10.1128/jb.172.7.3837-3841.1990;
RA Teather R.M., Erfle J.D.;
RT "DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase
RT (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene.";
RL J. Bacteriol. 172:3837-3841(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19169 / S85;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J.,
RA Stevenson D.M., Boyum J., Brumm P.I., Mead D.;
RT "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85.";
RL Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19169 / S85;
RA Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B.,
RA Russell J.B., Cann I.K.O., Mackie R.I., White B.A.;
RT "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85.";
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-
CC glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. {ECO:0000305}.
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DR EMBL; M33676; AAA24896.1; -; Genomic_DNA.
DR EMBL; CP001792; ACX76541.1; -; Genomic_DNA.
DR EMBL; CP002158; ADL24776.1; -; Genomic_DNA.
DR PIR; A44507; A44507.
DR RefSeq; WP_014545066.1; NC_017448.1.
DR PDB; 1MVE; X-ray; 1.70 A; A=25-266.
DR PDB; 1ZM1; X-ray; 2.30 A; A/B=27-267.
DR PDB; 2R49; X-ray; 2.20 A; A=26-266.
DR PDB; 3AXD; X-ray; 1.53 A; A/B=25-271.
DR PDB; 3AXE; X-ray; 1.53 A; A=25-271.
DR PDB; 3H0O; X-ray; 1.40 A; A=27-266.
DR PDB; 3HR9; X-ray; 1.70 A; A=26-266.
DR PDBsum; 1MVE; -.
DR PDBsum; 1ZM1; -.
DR PDBsum; 2R49; -.
DR PDBsum; 3AXD; -.
DR PDBsum; 3AXE; -.
DR PDBsum; 3H0O; -.
DR PDBsum; 3HR9; -.
DR AlphaFoldDB; P17989; -.
DR SMR; P17989; -.
DR STRING; 59374.Fisuc_2961; -.
DR CAZy; GH16; Glycoside Hydrolase Family 16.
DR PRIDE; P17989; -.
DR EnsemblBacteria; ADL24776; ADL24776; FSU_0226.
DR KEGG; fsc:FSU_0226; -.
DR KEGG; fsu:Fisuc_2961; -.
DR eggNOG; COG2273; Bacteria.
DR HOGENOM; CLU_063230_0_0_0; -.
DR OMA; WQLATHT; -.
DR OrthoDB; 889932at2; -.
DR BRENDA; 3.2.1.73; 771.
DR EvolutionaryTrace; P17989; -.
DR Proteomes; UP000000517; Chromosome.
DR GO; GO:0042972; F:licheninase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR044791; Beta-glucanase/XTH.
DR InterPro; IPR008264; Beta_glucanase.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR000757; GH16.
DR InterPro; IPR008263; GH16_AS.
DR PANTHER; PTHR31062; PTHR31062; 1.
DR Pfam; PF00722; Glyco_hydro_16; 1.
DR PRINTS; PR00737; GLHYDRLASE16.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS01034; GH16_1; 1.
DR PROSITE; PS51762; GH16_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycosidase; Hydrolase;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000269|PubMed:2193918"
FT CHAIN 28..349
FT /note="Beta-glucanase"
FT /id="PRO_0000011793"
FT DOMAIN 28..197
FT /note="GH16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01098"
FT REPEAT 271..277
FT /note="1"
FT REPEAT 278..284
FT /note="2"
FT REPEAT 285..291
FT /note="3"
FT REPEAT 292..298
FT /note="4"
FT REPEAT 301..307
FT /note="5"
FT REGION 258..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..307
FT /note="5 X 7 AA tandem repeats of P-X-S-S-S-S-X"
FT COMPBIAS 267..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 79
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064"
FT ACT_SITE 83
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064"
FT STRAND 29..39
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 41..51
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 58..65
FT /evidence="ECO:0007829|PDB:3H0O"
FT TURN 67..70
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:2R49"
FT STRAND 79..85
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 91..100
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 109..112
FT /evidence="ECO:0007829|PDB:3H0O"
FT TURN 117..119
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 122..128
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 140..145
FT /evidence="ECO:0007829|PDB:3H0O"
FT HELIX 148..151
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 157..167
FT /evidence="ECO:0007829|PDB:3H0O"
FT HELIX 169..172
FT /evidence="ECO:0007829|PDB:3H0O"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 182..194
FT /evidence="ECO:0007829|PDB:3H0O"
FT TURN 196..198
FT /evidence="ECO:0007829|PDB:3H0O"
FT HELIX 200..202
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 204..211
FT /evidence="ECO:0007829|PDB:3H0O"
FT TURN 218..220
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 221..223
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:3H0O"
FT HELIX 237..239
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 240..243
FT /evidence="ECO:0007829|PDB:3H0O"
FT STRAND 246..253
FT /evidence="ECO:0007829|PDB:3H0O"
SQ SEQUENCE 349 AA; 37738 MW; 16DC4F5BDEFC578A CRC64;
MNIKKTAVKS ALAVAAAAAA LTTNVSAKDF SGAELYTLEE VQYGKFEARM KMAAASGTVS
SMFLYQNGSE IADGRPWVEV DIEVLGKNPG SFQSNIITGK AGAQKTSEKH HAVSPAADQA
FHTYGLEWTP NYVRWTVDGQ EVRKTEGGQV SNLTGTQGLR FNLWSSESAA WVGQFDESKL
PLFQFINWVK VYKYTPGQGE GGSDFTLDWT DNFDTFDGSR WGKGDWTFDG NRVDLTDKNI
YSRDGMLILA LTRKGQESFN GQVPRDDEPA PQSSSSAPAS SSSVPASSSS VPASSSSAFV
PPSSSSATNA IHGMRTTPAV AKEHRNLVNA KGAKVNPNGH KRYRVNFEH
