ID   GUB_NIACI               Reviewed;         409 AA.
AC   P19254;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Beta-glucanase;
DE            EC=3.2.1.73;
DE   AltName: Full=Endo-beta-1,3-1,4 glucanase;
DE   Flags: Precursor;
GN   Name=bgc;
OS   Niallia circulans (Bacillus circulans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
OX   NCBI_TaxID=1397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WL-12;
RX   PubMed=2377467; DOI=10.1093/nar/18.14.4248;
RA   Bueno A., Vasquez de Aldana C.R., Correa J., del Rey F.;
RT   "Nucleotide sequence of a 1,3-1,4-beta-glucanase-encoding gene in Bacillus
RT   circulans WL-12.";
RL   Nucleic Acids Res. 18:4248-4248(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-
CC         glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 8 (cellulase D) family.
CC       {ECO:0000305}.
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DR   EMBL; X52880; CAA37062.1; -; Genomic_DNA.
DR   PIR; S10485; S10485.
DR   AlphaFoldDB; P19254; -.
DR   SMR; P19254; -.
DR   CAZy; GH8; Glycoside Hydrolase Family 8.
DR   BRENDA; 3.2.1.73; 649.
DR   GO; GO:0042972; F:licheninase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR002037; Glyco_hydro_8.
DR   InterPro; IPR019834; Glyco_hydro_8_CS.
DR   Pfam; PF01270; Glyco_hydro_8; 1.
DR   PRINTS; PR00735; GLHYDRLASE8.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS00812; GLYCOSYL_HYDROL_F8; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..409
FT                   /note="Beta-glucanase"
FT                   /id="PRO_0000007929"
FT   ACT_SITE        95
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        156
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10058"
SQ   SEQUENCE   409 AA;  44816 MW;  C7561216F7D13E76 CRC64;
     MRKNRGFSFS SKAVMMCCLA FLLIPASFAF AAPNKPFPQH TTYTSGSIKP NHVTQSAMDS
     SVKAKWDSWK SAYLKTAGTG KYYVKYQSNG DTVSEAHGYG MLATVIMAGY DGNAQTYFDG
     LYQYYKAHPS ANNSKLMAWK QNSSFQNIEG ADSATDGDMD IAYSLLLADK QWGSSGSINY
     LQAGKDIINA IMQSDVNQSQ WTLRLGDWAT DNTFKNATRP SDFMLNHLKA FQAATGDARW
     ANVIDKTYTI INSLYSGYSS STGLLPDFVV LSGSTYKPAS ADFLEGANDG SYDYNSCRTP
     WRIATDYLMT GDSRALNQLN QMNSWISAKV SGNPSNVKDG YKLNGTVTGS GGSGAFYAPF
     GVSAMTSSVN QNWLNSVWTK TAGSSNEGYY EDSIKLFSMI VMSGNWWTY