AMT1_HALMT
ID AMT1_HALMT Reviewed; 516 AA.
AC I3R0S7; B8ZYW2;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Ammonium transporter Amt1 {ECO:0000305};
GN Name=amt1 {ECO:0000303|Ref.3};
GN Synonyms=amtB1 {ECO:0000303|PubMed:24039236};
GN OrderedLocusNames=HFX_0095 {ECO:0000312|EMBL:AFK17837.1};
GN ORFNames=BM92_08805 {ECO:0000312|EMBL:AHZ22737.1};
OS Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=523841;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=22843593; DOI=10.1128/jb.00880-12;
RA Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA Zhou J., Hu S., Xiang H.;
RT "Complete genome sequence of the metabolically versatile halophilic
RT archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT hydroxyvalerate) producer.";
RL J. Bacteriol. 194:4463-4464(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA Bautista V.;
RT "Transcriptional profiles of Haloferax mediterranei on the basis of
RT nitrogen availability.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-516.
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA Camacho M., Pedro-Roig L., Bonete M.J.;
RT "Localization of an ammonium transporter protein from Haloferax
RT mediterranei.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH GLNK1 AND GLNK2, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=24039236; DOI=10.1002/mbo3.120;
RA Pedro-Roig L., Lange C., Bonete M.J., Soppa J., Maupin-Furlow J.;
RT "Nitrogen regulation of protein-protein interactions and transcript levels
RT of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea.";
RL MicrobiologyOpen 2:826-840(2013).
CC -!- FUNCTION: Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3))
CC (By similarity). Transport is electrogenic (By similarity).
CC {ECO:0000250|UniProtKB:O29285}.
CC -!- SUBUNIT: Homotrimer (By similarity). Interacts with both GlnK1 and
CC GlnK2 after ammonium shock (PubMed:24039236). Interaction is rapid,
CC reversible and dependent on nitrogen source (PubMed:24039236).
CC {ECO:0000250|UniProtKB:O29285, ECO:0000269|PubMed:24039236}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24039236};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Highly expressed during ammonium starvation under nitrate-
CC rich conditions. {ECO:0000269|PubMed:24039236}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; CP001868; AFK17837.1; -; Genomic_DNA.
DR EMBL; CP007551; AHZ22737.1; -; Genomic_DNA.
DR EMBL; FM991873; CAX20372.1; -; Genomic_DNA.
DR AlphaFoldDB; I3R0S7; -.
DR SMR; I3R0S7; -.
DR STRING; 523841.HFX_0095; -.
DR TCDB; 1.A.11.2.11; the ammonium transporter channel (amt) family.
DR EnsemblBacteria; AFK17837; AFK17837; HFX_0095.
DR EnsemblBacteria; AHZ22737; AHZ22737; BM92_08805.
DR KEGG; hme:HFX_0095; -.
DR eggNOG; arCOG04397; Archaea.
DR HOGENOM; CLU_000445_33_1_2; -.
DR OMA; IWLSIMS; -.
DR OrthoDB; 66605at2157; -.
DR Proteomes; UP000006469; Chromosome.
DR Proteomes; UP000027075; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..516
FT /note="Ammonium transporter Amt1"
FT /id="PRO_0000453008"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 426..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..489
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 179
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
FT SITE 346
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
FT CONFLICT 441..464
FT /note="Missing (in Ref. 3; CAX20372)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 516 AA; 52311 MW; FACCBC050617DA15 CRC64;
MIPLQVDPNV VAQGVNYVWI LVVSFLIFFM QPGFALLEAG QVRAKNVGNV LMKNMTDWAL
GVLVYFVVGA GVATIVGGLT SPGGFDVAAA FSYIGDSGAW IDWLFGAVFA MTAATIVSGA
VAERMDFRAY VVFAATITGF IYPVVQGLTW SGGLLSGSGY LGAALGVGYL DFAGATVVHM
CGGVAGLVGA KMVGPRKGRF GASGESQPIP GHSMLLAVLG TLILAFGWYG FNVGTQATVL
ATTESGGLEF MGAALGRVAL VTTLGMGAGA VAAMVVSTNY QGKPDPLWMA NGLLAGLVAV
TGAVPHVTWW GGLVLGALGG AIVLPAYRWT VDSLKIDDVC GVFAVHGVAG AVGTALIPVF
AVGGFSATQL VMQVAGVGII ALWTIVASAV VFAAAGTVFG LRVSEEEELE GLDIGEHGVS
VYPEFIGESG PDRGVGTRAA TDGGNDVRTD GGNDVRTDGG NDVRTDGGND VRTDGGNDVR
TDGGNDVRTD GDVVGDNGVA VTEGNDSAAV DGGENQ
