ID   GUB_NICPL               Reviewed;         370 AA.
AC   P07979;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 3.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Lichenase;
DE            EC=3.2.1.73;
DE   AltName: Full=Endo-beta-1,3-1,4 glucanase;
DE   Flags: Precursor;
GN   Name=GN1;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. P2;
RX   PubMed=3240865; DOI=10.1016/0378-1119(88)90100-x;
RA   de Loose M., Alliotte T., Gheysen G., Genetello C., Gielen J., Soetaert P.,
RA   van Montagu M., Inze D.;
RT   "Primary structure of a hormonally regulated beta-glucanase of Nicotiana
RT   plumbaginifolia.";
RL   Gene 70:13-23(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2152158; DOI=10.2307/3869334;
RA   Castresana C., de Carvalho F., Gheysen G., Habets M., Inze D.,
RA   van Montagu M.;
RT   "Tissue-specific and pathogen-induced regulation of a Nicotiana
RT   plumbaginifolia beta-1,3-glucanase gene.";
RL   Plant Cell 2:1131-1143(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-
CC         glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73;
CC   -!- PATHWAY: Glycan metabolism; beta-D-glucan degradation.
CC   -!- MISCELLANEOUS: The expression of beta-glucanase is influenced by auxins
CC       and cytokinins.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; X07280; CAA30261.1; -; mRNA.
DR   EMBL; M63634; AAA34078.1; -; Genomic_DNA.
DR   PIR; JQ0982; JQ0982.
DR   PIR; S03209; S03209.
DR   AlphaFoldDB; P07979; -.
DR   SMR; P07979; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   UniPathway; UPA00350; -.
DR   GO; GO:0042972; F:licheninase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; PTHR32227; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase; Hydrolase; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..370
FT                   /note="Lichenase"
FT                   /id="PRO_0000011898"
FT   ACT_SITE        126
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        271
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   CONFLICT        164
FT                   /note="S -> T (in Ref. 2; AAA34078)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        199
FT                   /note="R -> G (in Ref. 2; AAA34078)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        250
FT                   /note="S -> L (in Ref. 2; AAA34078)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   370 AA;  40599 MW;  CB359DFD188F84C1 CRC64;
     MDTSHKHIAL QMAAIILLGL LVSSTEIVGA QSVGVCYGML GNNLPPASQV VQLYKSKNIR
     RMRLYDPNQA ALQALRGSNI EVMLGVPNSD LQNIAANPSN ANNWVQRNVR NFWPAVKFRY
     IAVGNEVSPV TGTSSLTRYL LPAMRNIRNA ISSAGLQNNI KVSSSVDMTL IGNSFPPSQG
     SFRNDVRSFI DPIIGFVRRI NSPLLVNIYP YFSYAGNPRD ISLPYALFTA PNVVVQDGSL
     GYRNLFDAMS DAVYAALSRA GGGSIEIVVS ESGWPSAGAF AATTNNAATY YKNLIQHVKR
     GSPRRPNKVI ETYLFAMFDE NNKNPELEKH FGLFSPNKQP KYPLSFGFSD RYWDISAENN
     ATAASLISEM