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AMT1_METJA
ID   AMT1_METJA              Reviewed;         391 AA.
AC   Q60366;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Ammonium transporter Amt1 {ECO:0000305};
GN   Name=amt1 {ECO:0000303|PubMed:17203075}; OrderedLocusNames=MJ0058;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   ACTIVITY REGULATION, SUBUNIT, AND INTERACTION WITH GLNK1.
RX   PubMed=17203075; DOI=10.1038/sj.emboj.7601492;
RA   Yildiz O., Kalthoff C., Raunser S., Kuhlbrandt W.;
RT   "Structure of GlnK1 with bound effectors indicates regulatory mechanism for
RT   ammonia uptake.";
RL   EMBO J. 26:589-599(2007).
CC   -!- FUNCTION: Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3))
CC       (By similarity). Transport is electrogenic (By similarity).
CC       {ECO:0000250|UniProtKB:O29285}.
CC   -!- ACTIVITY REGULATION: Activity is regulated by the nitrogen regulatory
CC       protein GlnK1 via direct interaction (PubMed:17203075). Formation of
CC       the GlnK1/Amt1 complex is decreased in the presence of Mg-ATP or 2-
CC       oxoglutarate. The presence of both effectors abolishes the formation of
CC       the complex (PubMed:17203075). {ECO:0000269|PubMed:17203075}.
CC   -!- SUBUNIT: Homotrimer (PubMed:17203075). Interacts and forms a complex
CC       with GlnK1 (PubMed:17203075). {ECO:0000269|PubMed:17203075}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98038.1; -; Genomic_DNA.
DR   PIR; B64307; B64307.
DR   AlphaFoldDB; Q60366; -.
DR   SMR; Q60366; -.
DR   STRING; 243232.MJ_0058; -.
DR   EnsemblBacteria; AAB98038; AAB98038; MJ_0058.
DR   KEGG; mja:MJ_0058; -.
DR   eggNOG; arCOG04397; Archaea.
DR   HOGENOM; CLU_000445_33_1_2; -.
DR   InParanoid; Q60366; -.
DR   OMA; CAGSDVM; -.
DR   PhylomeDB; Q60366; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR   GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.10.3430.10; -; 1.
DR   InterPro; IPR029020; Ammonium/urea_transptr.
DR   InterPro; IPR001905; Ammonium_transpt.
DR   InterPro; IPR018047; Ammonium_transpt_CS.
DR   InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR   Pfam; PF00909; Ammonium_transp; 1.
DR   TIGRFAMs; TIGR00836; amt; 1.
DR   PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE   1: Evidence at protein level;
KW   Ammonia transport; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..391
FT                   /note="Ammonium transporter Amt1"
FT                   /id="PRO_0000139764"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..132
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        192..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..358
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   SITE            158
FT                   /note="Twin-His motif. Important for optimum substrate
FT                   conductance"
FT                   /evidence="ECO:0000250|UniProtKB:P69681"
FT   SITE            309
FT                   /note="Twin-His motif. Important for optimum substrate
FT                   conductance"
FT                   /evidence="ECO:0000250|UniProtKB:P69681"
SQ   SEQUENCE   391 AA;  41153 MW;  33632A73849E1C9A CRC64;
     MFEVKHMDGI DVFFFMWAAS LIFFMKAGFI ALEIGQFRAK NVSYHCVLKL LDLAAVFIAY
     LFIGYGISYG FENIMPLITG TFDADLGAWW MKMVMFAAAA VTIITGGVAE RIKILPYFIG
     ALIVGGILYP IVEHLVWGGG FANLGINFHD YAGSGAVHLF GGLVGLMAAY VLGPRIDKYI
     NGKPQAIPGH NIPIAVLGAF ILAFGWYGFN IGSASGIANG VELASVAMAT TMALAGGIIG
     GALSSRNDPL YTANGMCAGL VAVCSGVDLF TPIGAFIVGL LAGIQQPFTY KFIEEKLKID
     DVCAIGPVHA MSGLIGVICA GIPFLLKADA VSKVSITGQI IGAIVIALIA IVGGLIIYKG
     LDLTIGLRVS EEAEKVGLDT AILQTTAYSE E
 
 
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