ID   GUC2B_DIDVI             Reviewed;         109 AA.
AC   Q28358;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Guanylate cyclase activator 2B;
DE   Contains:
DE     RecName: Full=Uroguanylin;
DE              Short=UGN;
DE   Flags: Precursor;
GN   Name=GUCA2B;
OS   Didelphis virginiana (North American opossum) (Didelphis marsupialis
OS   virginiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Didelphimorphia; Didelphidae; Didelphis.
OX   NCBI_TaxID=9267;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Colon;
RX   PubMed=8605009; DOI=10.1006/bbrc.1996.0255;
RA   Fan X., Hamra F.K., Freeman R.H., Eber S.L., Krause W.J., Lim R.W.,
RA   Pace V.M., Currie M.G., Forte L.R.;
RT   "Uroguanylin: cloning of preprouroguanylin cDNA, mRNA expression in the
RT   intestine and heart and isolation of uroguanylin and prouroguanylin from
RT   plasma.";
RL   Biochem. Biophys. Res. Commun. 219:457-462(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF 95-109.
RC   TISSUE=Urine;
RX   PubMed=7902563; DOI=10.1073/pnas.90.22.10464;
RA   Hamra F.K., Forte L.R., Eber S.L., Pidhorodeckyj N.V., Krause W.J.,
RA   Freeman R.H., Chin D.T., Tompkins J.A., Fok K.F., Smith C.E., Duffin K.L.,
RA   Siegel N.R., Currie M.G.;
RT   "Uroguanylin: structure and activity of a second endogenous peptide that
RT   stimulates intestinal guanylate cyclase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:10464-10468(1993).
CC   -!- FUNCTION: Endogenous activator of intestinal guanylate cyclase. It
CC       stimulates this enzyme through the same receptor binding region as the
CC       heat-stable enterotoxins. May be a potent physiological regulator of
CC       intestinal fluid and electrolyte transport. May be an
CC       autocrine/paracrine regulator of intestinal salt and water transport.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Small and large intestine and atria and ventricles
CC       of heart. Both uroguanylin and prouroguanylin are found in plasma.
CC   -!- SIMILARITY: Belongs to the guanylin family. {ECO:0000305}.
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DR   EMBL; U49353; AAB00553.1; -; mRNA.
DR   AlphaFoldDB; Q28358; -.
DR   SMR; Q28358; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030250; F:guanylate cyclase activator activity; IEA:InterPro.
DR   Gene3D; 3.90.1450.10; -; 1.
DR   InterPro; IPR000879; Guanylin.
DR   InterPro; IPR036382; Guanylin_sf.
DR   PANTHER; PTHR11318; PTHR11318; 1.
DR   Pfam; PF02058; Guanylin; 1.
DR   PIRSF; PIRSF001849; Guanylin; 1.
DR   PRINTS; PR00774; GUANYLIN.
DR   SUPFAM; SSF89890; SSF89890; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..94
FT                   /evidence="ECO:0000269|PubMed:7902563"
FT                   /id="PRO_0000013145"
FT   PEPTIDE         95..109
FT                   /note="Uroguanylin"
FT                   /id="PRO_0000013146"
FT   DISULFID        65..78
FT                   /evidence="ECO:0000250"
FT   DISULFID        98..106
FT                   /evidence="ECO:0000250"
FT   DISULFID        101..109
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   109 AA;  12040 MW;  AE948E210CA3AE7A CRC64;
     MKVLALPVAV AAMLLVLAQN TQSVYIQYEG FQVKLDSVKK LDELLEQPRS FRHRMGTQRD
     PSVLCSDPAL PSDLQPVCEN SQAANIFRAL RSISQEDCEL CINVACTGC