AMT23_MEDTR
ID AMT23_MEDTR Reviewed; 481 AA.
AC G7LAA8; A0A0C3Y356; A0A396GQY3; A0A396GT18;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 2.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Ammonium transporter 2 member 3 {ECO:0000303|PubMed:25841038};
DE Short=Ammonium transporter 2;3 {ECO:0000303|PubMed:25841038};
DE Short=MtAMT2;3 {ECO:0000303|PubMed:25841038};
GN Name=AMT2-3 {ECO:0000303|PubMed:25841038};
GN OrderedLocusNames=MTR_8g074750 {ECO:0000312|EMBL:AET03699.2};
GN ORFNames=MtrunA17_Chr8g0371161 {ECO:0000312|EMBL:RHN41914.1},
GN MtrunA17_Chr8g0371171 {ECO:0000312|EMBL:RHN41915.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY ARBUSCULAR MYCORRHIZAL FUNGI,
RP DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=25841038; DOI=10.1105/tpc.114.131144;
RA Breuillin-Sessoms F., Floss D.S., Gomez S.K., Pumplin N., Ding Y.,
RA Levesque-Tremblay V., Noar R.D., Daniels D.A., Bravo A., Eaglesham J.B.,
RA Benedito V.A., Udvardi M.K., Harrison M.J.;
RT "Suppression of arbuscule degeneration in Medicago truncatula phosphate
RT transporter4 mutants is dependent on the ammonium transporter 2 family
RT protein AMT2;3.";
RL Plant Cell 27:1352-1366(2015).
CC -!- FUNCTION: Involved in ammonium transport (By similarity). Required for
CC arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus
CC versiforme and G.intraradices) in low nitrogen conditions
CC (PubMed:25841038). {ECO:0000250|UniProtKB:Q9M6N7,
CC ECO:0000269|PubMed:25841038}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25841038};
CC Multi-pass membrane protein {ECO:0000255}. Note=Present on the
CC periarbuscular membrane in cells containing arbuscules during
CC arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus
CC versiforme). {ECO:0000269|PubMed:25841038}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in mycorrhizal roots
CC (PubMed:25841038). Also observed in the cortex and endodermis of non-
CC mycorrhizal roots (PubMed:25841038). {ECO:0000269|PubMed:25841038}.
CC -!- DEVELOPMENTAL STAGE: Expressed in cortical cells containing arbuscules
CC of mycorrhizal roots upon arbuscular mycorrhizal (AM) symbiosis with AM
CC fungi (e.g. Glomus intraradices). {ECO:0000269|PubMed:25841038}.
CC -!- INDUCTION: Accumulates in roots during colonization by arbuscular
CC mycorrhizal (AM) fungi (e.g. Glomus versiforme).
CC {ECO:0000269|PubMed:25841038}.
CC -!- DISRUPTION PHENOTYPE: Normal arbuscular mycorrhizal (AM) symbiosis with
CC AM fungi (PubMed:25841038). Plants missing both PT4 and AMT2-3 fail to
CC establish AM symbiosis with AM fungi in high nitrogen conditions,
CC leading to premature arbuscule degeneration (PAD); these phenotypes are
CC not suppressed in nitrogen-deprived conditions (PubMed:25841038).
CC {ECO:0000269|PubMed:25841038}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=RHN41914.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=RHN41915.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CM001224; AET03699.2; -; Genomic_DNA.
DR EMBL; PSQE01000008; RHN41914.1; ALT_SEQ; Genomic_DNA.
DR EMBL; PSQE01000008; RHN41915.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_003629223.2; XM_003629175.2.
DR AlphaFoldDB; G7LAA8; -.
DR SMR; G7LAA8; -.
DR STRING; 3880.AET03699; -.
DR EnsemblPlants; AET03699; AET03699; MTR_8g074750.
DR GeneID; 11444316; -.
DR Gramene; AET03699; AET03699; MTR_8g074750.
DR KEGG; mtr:MTR_8g074750; -.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_4_1; -.
DR OrthoDB; 910733at2759; -.
DR Proteomes; UP000002051; Chromosome 8.
DR Proteomes; UP000265566; Chromosome 8.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0085042; C:periarbuscular membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..481
FT /note="Ammonium transporter 2 member 3"
FT /id="PRO_0000450041"
FT TOPO_DOM 1..36
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 37..57
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 63..83
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..123
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 124..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 152..172
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..184
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 185..205
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 221..241
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..248
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 249..269
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 282..302
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..304
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 305..325
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..338
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 339..359
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360..363
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 364..381
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..395
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 396..416
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..481
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 481 AA; 53080 MW; E44CDEAD05A0C22C CRC64;
MNFNSSKYIS HLPESLLPND ASPEWNNKAD NAWQLTAATL VGLQTVPGLV ILYGSMVKKK
WAVNSAFMAL YAFAAVLVCW VLWAHHMAFG TKLLPFVGKP NFALSQKFLL SKASTNYYLP
MADFVFYQFA FAAITLVLLG GSLLGRMNFY AWMLFVPLWL TLSYTVGAFT IWGNGFLEGK
IIDYAGGFVI HLSSGVAGFT AAYWVGPRTS NDRQNFPPNN IIHMLGGAGF LWMGWTGFNG
GAPFQVGEIT SLAIFNTHLC TATSILVWIS LDMAVYKKGS LIGSVQGMMT GLVCITPGAG
LVDPWAAILM GALSGSIPWY TMMVLHKKSP FFQSVDDTLG VFHTHAVAGI LGGILSGVFA
KPKLLRILYG PYGSGLLYSY FDDNIGQGIK QMWYQLLGAV FITIWNVVIT SLICILLNRF
VNLRMQEEDL EVGDDAAHGE EAYVLWGDGE RMRLPLRRDI SPIIPYISHQ RHSFPINKID
E
