GUDP_BACSU
ID GUDP_BACSU Reviewed; 455 AA.
AC P42237;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Probable galactarate/D-glucarate transporter GudP {ECO:0000305};
GN Name=gudP {ECO:0000303|PubMed:12044674}; Synonyms=ycbE;
GN OrderedLocusNames=BSU02480;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT region of the Bacillus subtilis chromosome.";
RL Microbiology 141:269-275(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 167.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP POSSIBLE FUNCTION, AND INDUCTION.
RX PubMed=12044674; DOI=10.1111/j.1574-6968.2002.tb11180.x;
RA Hosoya S., Yamane K., Takeuchi M., Sato T.;
RT "Identification and characterization of the Bacillus subtilis D-
RT glucarate/galactarate utilization operon ycbCDEFGHJ.";
RL FEMS Microbiol. Lett. 210:193-199(2002).
CC -!- FUNCTION: Probably involved in the uptake of galactarate and/or D-
CC glucarate. {ECO:0000305|PubMed:12044674}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=galactarate(in) + H(+)(in) = galactarate(out) + H(+)(out);
CC Xref=Rhea:RHEA:28478, ChEBI:CHEBI:15378, ChEBI:CHEBI:16537;
CC Evidence={ECO:0000305|PubMed:12044674};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucarate(in) + H(+)(in) = D-glucarate(out) + H(+)(out);
CC Xref=Rhea:RHEA:28474, ChEBI:CHEBI:15378, ChEBI:CHEBI:30612;
CC Evidence={ECO:0000305|PubMed:12044674};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced in the presence of D-glucarate or D-galactarate.
CC {ECO:0000269|PubMed:12044674}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Phthalate
CC permease family. {ECO:0000305}.
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DR EMBL; D30808; BAA06469.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12042.2; -; Genomic_DNA.
DR PIR; H69752; H69752.
DR RefSeq; NP_388130.2; NC_000964.3.
DR RefSeq; WP_003234818.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P42237; -.
DR SMR; P42237; -.
DR STRING; 224308.BSU02480; -.
DR TCDB; 2.A.1.14.1; the major facilitator superfamily (mfs).
DR PaxDb; P42237; -.
DR PRIDE; P42237; -.
DR EnsemblBacteria; CAB12042; CAB12042; BSU_02480.
DR GeneID; 938413; -.
DR KEGG; bsu:BSU02480; -.
DR PATRIC; fig|224308.179.peg.255; -.
DR eggNOG; COG2271; Bacteria.
DR InParanoid; P42237; -.
DR OMA; LWWAFYH; -.
DR PhylomeDB; P42237; -.
DR BioCyc; BSUB:BSU02480-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000849; Sugar_P_transporter.
DR Pfam; PF07690; MFS_1; 2.
DR PIRSF; PIRSF002808; Hexose_phosphate_transp; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..455
FT /note="Probable galactarate/D-glucarate transporter GudP"
FT /id="PRO_0000121384"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 167
FT /note="S -> P (in Ref. 1; BAA06469)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 49244 MW; A069905BC7922757 CRC64;
MKKDFASVTP AGKKTSVRWF IVFMLFLVTS INYADRATLS ITGDSVQHDL GLDSVAMGYV
FSAFGWAYVI GQLPGGWLLD RFGSKTIIAL SIFFWSFFTL LQGAIGFFSA GTAIILLFAL
RFLVGLSEAP SFPGNGRVVA SWFPSSERGT ASAFFNSAQY FAIVIFSPLM GWLTHSFGWH
SVFVVMGIAG ILLAVIWLKT VYEPKKHPKV NEAELAYIEQ GGGLISMDDS KSKQETESKW
PYIKQLLTNR MLIGVYIAQY CITTLTYFFL TWFPVYLVQA RGMSILEAGF VASLPALCGF
AGGVLGGIVS DILLKKGRSL TFARKVPIIA GMLLSCSMIV CNYTDSAWLV VVIMSLAFFG
KGFGALGWAV VSDTSPKECA GLSGGLFNTF GNIASITTPI IIGYIVNATG SFNGALVFVG
ANAIAAILSY LLLVGPIKRV VLKKQEQDPD QSLPV
