GUDU_DROME
ID GUDU_DROME Reviewed; 669 AA.
AC Q9VM21; Q6NNW8;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Armadillo repeat-containing protein gudu {ECO:0000303|PubMed:24055424};
GN Name=gudu {ECO:0000303|PubMed:24055424, ECO:0000312|FlyBase:FBgn0031905};
GN ORFNames=CG5155 {ECO:0000312|FlyBase:FBgn0031905};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAR82832.1, ECO:0000312|EMBL:ACS12722.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAR82832.1};
RA Stapleton M., Booth B., Brokstein P., Hong L., Agbayani A., Carlson J.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R., Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Sandler J., Wan K., Yu C., Lewis S.E.,
RA Rubin G.M., Celniker S.;
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=24055424; DOI=10.1016/j.gene.2013.08.080;
RA Cheng W., Ip Y.T., Xu Z.;
RT "Gudu, an Armadillo repeat-containing protein, is required for
RT spermatogenesis in Drosophila.";
RL Gene 531:294-300(2013).
CC -!- FUNCTION: Important for spermatogenesis where it may have a role in
CC sperm individualization. {ECO:0000269|PubMed:24055424}.
CC -!- TISSUE SPECIFICITY: Highly expressed in testis.
CC {ECO:0000269|PubMed:24055424}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown severely impairs male
CC fertility. Morphology of the sperm individualization complex is highly
CC abnormal. Seminal vesicles contain very few mature spermatozoa, which
CC are often bundled together. Female fertility is not affected.
CC {ECO:0000269|PubMed:24055424}.
CC -!- MISCELLANEOUS: Gudu is Chinese for 'alone without progeny' and refers
CC to the male infertility phenotype. {ECO:0000303|PubMed:24055424}.
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DR EMBL; AE014134; AAF52507.1; -; Genomic_DNA.
DR EMBL; BT011164; AAR82832.1; -; mRNA.
DR EMBL; BT088789; ACS12722.1; -; mRNA.
DR RefSeq; NP_609111.1; NM_135267.3.
DR AlphaFoldDB; Q9VM21; -.
DR SMR; Q9VM21; -.
DR IntAct; Q9VM21; 4.
DR STRING; 7227.FBpp0079066; -.
DR PaxDb; Q9VM21; -.
DR PRIDE; Q9VM21; -.
DR DNASU; 34014; -.
DR EnsemblMetazoa; FBtr0079438; FBpp0079066; FBgn0031905.
DR GeneID; 34014; -.
DR KEGG; dme:Dmel_CG5155; -.
DR UCSC; CG5155-RA; d. melanogaster.
DR CTD; 34014; -.
DR FlyBase; FBgn0031905; gudu.
DR VEuPathDB; VectorBase:FBgn0031905; -.
DR eggNOG; KOG0167; Eukaryota.
DR GeneTree; ENSGT00940000156625; -.
DR HOGENOM; CLU_028951_0_0_1; -.
DR InParanoid; Q9VM21; -.
DR OMA; TYMTSDN; -.
DR OrthoDB; 310809at2759; -.
DR PhylomeDB; Q9VM21; -.
DR SignaLink; Q9VM21; -.
DR BioGRID-ORCS; 34014; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34014; -.
DR PRO; PR:Q9VM21; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031905; Expressed in testis and 7 other tissues.
DR GO; GO:0007291; P:sperm individualization; IMP:FlyBase.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR Pfam; PF00514; Arm; 1.
DR SMART; SM00185; ARM; 9.
DR SUPFAM; SSF48371; SSF48371; 3.
PE 2: Evidence at transcript level;
KW Differentiation; Reference proteome; Repeat; Spermatogenesis.
FT CHAIN 1..669
FT /note="Armadillo repeat-containing protein gudu"
FT /id="PRO_0000439052"
FT REPEAT 100..139
FT /note="ARM 1"
FT /evidence="ECO:0000255"
FT REPEAT 141..180
FT /note="ARM 2"
FT /evidence="ECO:0000255"
FT REPEAT 240..279
FT /note="ARM 3"
FT /evidence="ECO:0000255"
FT REPEAT 281..320
FT /note="ARM 4"
FT /evidence="ECO:0000255"
FT REPEAT 322..365
FT /note="ARM 5"
FT /evidence="ECO:0000255"
FT REPEAT 367..406
FT /note="ARM 6"
FT /evidence="ECO:0000255"
FT REPEAT 408..447
FT /note="ARM 7"
FT /evidence="ECO:0000255"
FT REPEAT 492..531
FT /note="ARM 8"
FT /evidence="ECO:0000255"
FT REPEAT 574..613
FT /note="ARM 9"
FT /evidence="ECO:0000255"
FT REPEAT 615..654
FT /note="ARM 10"
FT /evidence="ECO:0000255"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 561
FT /note="M -> I (in Ref. 3; AAR82832)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 669 AA; 73181 MW; 312B10BB4EF2FDD9 CRC64;
MIGTSSGTSH NRSRKKKEQC GSCPNRFSKD KRQVAAEDSD TTEVESSTDE EERWKEVARA
AEIPADYYNI QKLVKYIKAG NQTATIVSLC CLKDYDLSTQ INQFAISDIG GLDVLVNILE
CSDTKCCLGA LKVLSDITLN IDIRKTIVDL DGIPLIVDIL NSSMKDLKTM AAETLANVCK
VRLARKYVRT CGGIPKLVDL IDIKLSILKT PRDQLSPDDL ESLDMTRAGA RALFTLADSK
HNMEQMRKSG IVPLMAQLLK SCHIDVVIPI MGTVRKCSSE PKFQLAITTE GMIPDIVSHL
SSENTELKME GSTAIYKCAF DGTTRDLVRE AGGLEPLVTI IKDKNVRENK PLLRGATGAI
WMCAVTDANV KVLDQLRTVN HLVALLNDEC DEVLTNVTGA ISECVRFQSN REQLRQAGGL
PAMVSLLNSS HAPLLENLAK GLKECAEDPD SMRILEDLDA VRLIWSLLKN PTTRVQAHAA
YAICPCVRNA NDSAELVRSL VGAMELVVGL LKSKDIMVLS AVCAAIATIA QDQTNLAILT
DLKVIYKLAD LVQTTDDLLR MNLAAAVAAC ACFGNNTEEL GRLRTVTPIV TYMTSDNPLV
HRSTAMALEK LSMDPQNCIT MHQSGVVPFL LECIGSTNKE LQLAAAGCLR NIRELALRAE
EYLLKIDDD
