AMT24_MEDTR
ID AMT24_MEDTR Reviewed; 480 AA.
AC G7L1W7;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Ammonium transporter 2 member 4 {ECO:0000303|PubMed:26511916};
DE Short=Ammonium transporter 2;4 {ECO:0000303|PubMed:26511916};
DE Short=MtAMT2;4 {ECO:0000303|PubMed:26511916};
GN Name=AMT2-4 {ECO:0000303|PubMed:26511916};
GN OrderedLocusNames=MTR_7g115050 {ECO:0000312|EMBL:AES82665.1};
GN ORFNames=MtrunA17_Chr7g0274111 {ECO:0000312|EMBL:RHN49401.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ARBUSCULAR MYCORRHIZAL
RP FUNGI.
RC STRAIN=cv. Jemalong A17;
RX PubMed=25841038; DOI=10.1105/tpc.114.131144;
RA Breuillin-Sessoms F., Floss D.S., Gomez S.K., Pumplin N., Ding Y.,
RA Levesque-Tremblay V., Noar R.D., Daniels D.A., Bravo A., Eaglesham J.B.,
RA Benedito V.A., Udvardi M.K., Harrison M.J.;
RT "Suppression of arbuscule degeneration in Medicago truncatula phosphate
RT transporter4 mutants is dependent on the ammonium transporter 2 family
RT protein AMT2;3.";
RL Plant Cell 27:1352-1366(2015).
RN [5]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Involved in ammonium transport (PubMed:25841038). May be
CC involved in arbuscular mycorrhizal (AM) symbiosis with AM fungi (By
CC similarity). {ECO:0000250|UniProtKB:G7LAA8,
CC ECO:0000269|PubMed:25841038}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9M6N7};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus
CC versiforme). {ECO:0000269|PubMed:25841038,
CC ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: Normal arbuscular mycorrhizal (AM) symbiosis with
CC AM fungi. {ECO:0000269|PubMed:25841038}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; CM001223; AES82665.1; -; Genomic_DNA.
DR EMBL; PSQE01000007; RHN49401.1; -; Genomic_DNA.
DR RefSeq; XP_003626447.1; XM_003626399.1.
DR AlphaFoldDB; G7L1W7; -.
DR SMR; G7L1W7; -.
DR STRING; 3880.AES82665; -.
DR EnsemblPlants; AES82665; AES82665; MTR_7g115050.
DR GeneID; 11423183; -.
DR Gramene; AES82665; AES82665; MTR_7g115050.
DR KEGG; mtr:MTR_7g115050; -.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_4_1; -.
DR OMA; CWVGWGF; -.
DR OrthoDB; 910733at2759; -.
DR Proteomes; UP000002051; Chromosome 7.
DR Proteomes; UP000265566; Chromosome 7.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0072488; P:ammonium transmembrane transport; IDA:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..480
FT /note="Ammonium transporter 2 member 4"
FT /id="PRO_0000450042"
FT TOPO_DOM 1..27
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 52..72
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..113
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 114..134
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..141
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 142..162
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..175
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 176..196
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 215..235
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..242
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 243..263
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..274
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 275..295
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..298
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 299..319
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 335..355
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..394
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 395..415
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..480
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 480 AA; 52393 MW; 15D1A593AFBE2D16 CRC64;
MELPSNLLPD EASPEWMNKG DNAWQLTAAT MVGLQSIPGL VILYGSLVKK TWAINSAFMA
FYAFASVLLC WVSWAYQMSF GEKMVFFLGK PNVALDEKFL LGKAFLGNFP NATMVFYQGV
FAGLTLILIA GALLGRMNIR AWMLFVPLWV TFSYTVVAFS IWCPDGWLAK RGVIDFAGGY
VIHLSAGVAG FTAAYWVGPR ADKDRETFPA ATNNMIMVLA GAGLLWMGWS GFNGGAPFVA
STIASLAILN THVCTAASIT VWVMLDTFYF GKPTVFGAVQ GMITGLVCIT PAAGVVQGWA
AILMGFISGS IPWYTMMVLH NKVNFLKKID DPMAVFHTHA IAGALGGILT GFFAVPKLCR
LFYMVPDWEK YIGLAYGLQN KGATQAGLKQ MVIQIEAIVF VICYNVLMTS LICLIVRVIV
PLRLNGDALQ MGDKAIHGED AFALHSEATK FVNIKRNQVY DTQDFSSIPE SRSLGELQMV
