AMT25_MEDTR
ID AMT25_MEDTR Reviewed; 481 AA.
AC A0A072VHJ1;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Ammonium transporter 2 member 5 {ECO:0000303|PubMed:26511916};
DE Short=Ammonium transporter 2;5 {ECO:0000303|PubMed:26511916};
DE Short=MtAMT2;5 {ECO:0000303|PubMed:26511916};
GN Name=AMT2-5 {ECO:0000303|PubMed:26511916};
GN OrderedLocusNames=MTR_1g036410 {ECO:0000312|EMBL:KEH40853.1};
GN ORFNames=MtrunA17_Chr1g0163571 {ECO:0000312|EMBL:RHN78262.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP INDUCTION BY ARBUSCULAR MYCORRHIZAL FUNGI.
RC STRAIN=cv. Jemalong A17;
RX PubMed=25841038; DOI=10.1105/tpc.114.131144;
RA Breuillin-Sessoms F., Floss D.S., Gomez S.K., Pumplin N., Ding Y.,
RA Levesque-Tremblay V., Noar R.D., Daniels D.A., Bravo A., Eaglesham J.B.,
RA Benedito V.A., Udvardi M.K., Harrison M.J.;
RT "Suppression of arbuscule degeneration in Medicago truncatula phosphate
RT transporter4 mutants is dependent on the ammonium transporter 2 family
RT protein AMT2;3.";
RL Plant Cell 27:1352-1366(2015).
RN [5]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Involved in ammonium transport (By similarity). May be
CC involved in arbuscular mycorrhizal (AM) symbiosis with AM fungi (By
CC similarity). {ECO:0000250|UniProtKB:G7LAA8,
CC ECO:0000250|UniProtKB:Q9M6N7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9M6N7};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus
CC versiforme). {ECO:0000269|PubMed:25841038,
CC ECO:0000269|PubMed:26511916}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; CM001217; KEH40853.1; -; Genomic_DNA.
DR EMBL; PSQE01000001; RHN78262.1; -; Genomic_DNA.
DR RefSeq; XP_013466812.1; XM_013611358.1.
DR AlphaFoldDB; A0A072VHJ1; -.
DR SMR; A0A072VHJ1; -.
DR EnsemblPlants; KEH40853; KEH40853; MTR_1g036410.
DR GeneID; 25482762; -.
DR Gramene; KEH40853; KEH40853; MTR_1g036410.
DR KEGG; mtr:MTR_1g036410; -.
DR HOGENOM; CLU_000445_33_4_1; -.
DR OrthoDB; 910733at2759; -.
DR Proteomes; UP000002051; Chromosome 1.
DR Proteomes; UP000265566; Chromosome 1.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..481
FT /note="Ammonium transporter 2 member 5"
FT /id="PRO_0000450043"
FT TOPO_DOM 1..28
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 29..49
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..116
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..141
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 142..162
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..180
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 181..201
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 216..236
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..250
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 251..271
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 276..296
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..299
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 300..320
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 334..354
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..393
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 394..414
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..481
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 481 AA; 52906 MW; 60098680261B7D5F CRC64;
MSGVPFPSNL LPSPSSPEWL SKADNAWQLM AATLVGMQSV PGLIILYGGA VKKKWAVNSA
FMSLYAFACV FFCWVFWAYR MSFGDTLFPF WGKPALALDE KYLFKQAFLG AFPNATMIYF
QCVFAAITLI LIAGAVLGRM NFYAWMMFVP LWLTFSYTFT AFSIWSTNGF LAKMGIIDYS
GGYVIHLSSG VAGFTAAYWV GPRLNKDRER FPPNNLLLML AGAGLLWMGW TGFNGGDPYS
VGLDASLAVL NTHACTATSL LTWVFLDVIF FRKPSVIGAV QGMITGLVCI TPAAGVVQGW
AALIMGLFSG SIPWFTMMVI HKRSKLLQKV DDTMAVLHTH AIAGSLGGIL TGLFAEPKLN
SLFYGVYNKY VGLFYGIQMN MASTGRRQIG IQLLGILFVI FVNVVSTSLI CLFIRLFVPL
RMSEEDMEIG DEAAHGEEAY AIWGQGDKHE NSNSKYGSSL YEDVEVAATT KNKRGSQIEM
M
