AMT2_DICDI
ID AMT2_DICDI Reviewed; 431 AA.
AC Q9BLG3; Q54YS9;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Ammonium transporter 2;
GN Name=amtB; ORFNames=DDB_G0277889;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX2;
RA Chen Y., Huang C.-H.;
RT "Cloning and characterization of three ammonium transporter genes in AX2
RT strain of Dictyostelium discoideum.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=17951519; DOI=10.1128/ec.00204-07;
RA Yoshino R., Morio T., Yamada Y., Kuwayama H., Sameshima M., Tanaka Y.,
RA Sesaki H., Iijima M.;
RT "Regulation of ammonia homeostasis by the ammonium transporter AmtA in
RT Dictyostelium discoideum.";
RL Eukaryot. Cell 6:2419-2428(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=14686953; DOI=10.1111/j.1432-0436.2003.07109002.x;
RA Follstaedt S.C., Kirsten J.H., Singleton C.K.;
RT "Temporal and spatial expression of ammonium transporter genes during
RT growth and development of Dictyostelium discoideum.";
RL Differentiation 71:557-566(2003).
RN [5]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=19108721; DOI=10.1186/1471-2121-9-71;
RA Kirsten J.H., Xiong Y., Davis C.T., Singleton C.K.;
RT "Subcellular localization of ammonium transporters in Dictyostelium
RT discoideum.";
RL BMC Cell Biol. 9:71-71(2008).
CC -!- FUNCTION: Ammonium transporter that mediates the excretion of ammonium
CC from the prestalk cells from the slug. Controls ammonium homeostasis
CC during growth and development. Ammonium has been shown to function as a
CC morphogen at multiple steps during the development.
CC {ECO:0000269|PubMed:14686953}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19108721};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19108721}. Endosome
CC membrane {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Lysosome membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Contractile vacuole membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000269|PubMed:19108721}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19108721}.
CC -!- DEVELOPMENTAL STAGE: Predominantly found in the prespores of the slug.
CC {ECO:0000269|PubMed:14686953}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; AF510717; AAP47146.1; -; mRNA.
DR EMBL; AB049068; BAB39710.1; -; Genomic_DNA.
DR EMBL; AAFI02000023; EAL68117.1; -; Genomic_DNA.
DR RefSeq; XP_642123.1; XM_637031.1.
DR AlphaFoldDB; Q9BLG3; -.
DR SMR; Q9BLG3; -.
DR STRING; 44689.DDB0214902; -.
DR TCDB; 1.A.11.2.9; the ammonium transporter channel (amt) family.
DR PaxDb; Q9BLG3; -.
DR EnsemblProtists; EAL68117; EAL68117; DDB_G0277889.
DR GeneID; 8621332; -.
DR KEGG; ddi:DDB_G0277889; -.
DR dictyBase; DDB_G0277889; amtB.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_1_1; -.
DR InParanoid; Q9BLG3; -.
DR OMA; MACWNSN; -.
DR PhylomeDB; Q9BLG3; -.
DR PRO; PR:Q9BLG3; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031164; C:contractile vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0036020; C:endolysosome membrane; IDA:dictyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; ISS:dictyBase.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:dictyBase.
DR GO; GO:0046903; P:secretion; IMP:dictyBase.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Cytoplasmic vesicle; Endosome; Lysosome;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..431
FT /note="Ammonium transporter 2"
FT /id="PRO_0000365574"
FT TOPO_DOM 1..19
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..101
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..162
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..206
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..246
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 277..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..300
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 301..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..378
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:19108721"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..431
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:19108721"
SQ SEQUENCE 431 AA; 46368 MW; F8C578EA381F22E8 CRC64;
MEYLELNGTK EGLSAAVDDM WVLNATYLVF YMQAGFCMLE AGVVRAKNAK SIIMKSIIDT
AIGSLLFWAL GFGLAFGNAD KASNPVIGTS HFFLINYQNL SFFAFQWAFC ATSITIVSGS
LAERVHVTSC LVYTIVMSAF IYPLSAHWVW SYNGWLRMIG FNGIIDFSGS IVVHIVGGCI
GLVGTYLVGP RIGRFDSESG KPKPLPGHSI TIYTLGAFII WYGFYGFNTG STLGISGGGI
AIASRSAVTM TIIACASCAT TLLAIKIKSG KYDVVKSVNS LLGGLVSSAA VCSLIDPWAA
FIIGCVTSFV YLGCSHLLIK LRIDDPLDSS AIHLGCGIWG ALSVGLFSTQ ENLSLVLKKS
TNVYGLFFGG GFEQLGIQLL GIIIVMAWCF FCASILFTIL KKFHLLRIEP TKELMGIDID
SAGGPAYQWD N
