AMT2_HALMT
ID AMT2_HALMT Reviewed; 456 AA.
AC B8ZYW3;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Ammonium transporter Amt2 {ECO:0000305};
GN Name=amt2 {ECO:0000303|Ref.1};
GN Synonyms=amtB2 {ECO:0000303|PubMed:24039236};
GN OrderedLocusNames=HFX_0093 {ECO:0000312|EMBL:AFK17835.1};
GN ORFNames=BM92_08820 {ECO:0000312|EMBL:AHZ22739.1},
GN C439_09925 {ECO:0000312|EMBL:EMA02892.1},
GN E6P09_03560 {ECO:0000312|EMBL:QCQ74396.1};
OS Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=523841;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA Camacho M., Pedro-Roig L., Bonete M.J.;
RT "Overexpression and characterization of an ammonium tranporter from
RT Haloferax mediterranei.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=22843593; DOI=10.1128/jb.00880-12;
RA Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA Zhou J., Hu S., Xiang H.;
RT "Complete genome sequence of the metabolically versatile halophilic
RT archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT hydroxyvalerate) producer.";
RL J. Bacteriol. 194:4463-4464(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA Bautista V.;
RT "Transcriptional profiles of Haloferax mediterranei on the basis of
RT nitrogen availability.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RA DasSarma S., DasSarma P., DasSarma S., Fomenkov A., Vincze T., Anton B.P.,
RA Roberts R.J.;
RT "Methylomes of two halophilic Archaea, Haloarcula marismortui and Haloferax
RT mediterranei.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RC STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX PubMed=24039236; DOI=10.1002/mbo3.120;
RA Pedro-Roig L., Lange C., Bonete M.J., Soppa J., Maupin-Furlow J.;
RT "Nitrogen regulation of protein-protein interactions and transcript levels
RT of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea.";
RL MicrobiologyOpen 2:826-840(2013).
CC -!- FUNCTION: Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3))
CC (By similarity). Transport is electrogenic (By similarity).
CC {ECO:0000250|UniProtKB:O29285}.
CC -!- SUBUNIT: Homotrimer (By similarity). Interacts with both GlnK1 and
CC GlnK2 after ammonium shock (By similarity).
CC {ECO:0000250|UniProtKB:I3R0S7, ECO:0000250|UniProtKB:O29285}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expressed during ammonium starvation under nitrate-rich
CC conditions. {ECO:0000269|PubMed:24039236}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; FM991874; CAX20373.1; -; Genomic_DNA.
DR EMBL; CP001868; AFK17835.1; -; Genomic_DNA.
DR EMBL; AOLO01000007; EMA02892.1; -; Genomic_DNA.
DR EMBL; CP007551; AHZ22739.1; -; Genomic_DNA.
DR EMBL; CP039139; QCQ74396.1; -; Genomic_DNA.
DR RefSeq; WP_004058650.1; NZ_CP007551.1.
DR AlphaFoldDB; B8ZYW3; -.
DR SMR; B8ZYW3; -.
DR STRING; 523841.HFX_0093; -.
DR EnsemblBacteria; AFK17835; AFK17835; HFX_0093.
DR EnsemblBacteria; AHZ22739; AHZ22739; BM92_08820.
DR EnsemblBacteria; EMA02892; EMA02892; C439_09925.
DR EnsemblBacteria; QCQ74396; QCQ74396; E6P09_03560.
DR GeneID; 13026263; -.
DR GeneID; 40155463; -.
DR KEGG; hme:HFX_0093; -.
DR PATRIC; fig|523841.21.peg.2018; -.
DR eggNOG; arCOG04397; Archaea.
DR HOGENOM; CLU_000445_33_1_2; -.
DR OMA; IFFMHAG; -.
DR OrthoDB; 66605at2157; -.
DR Proteomes; UP000006469; Chromosome.
DR Proteomes; UP000011603; Unassembled WGS sequence.
DR Proteomes; UP000027075; Chromosome.
DR Proteomes; UP000299011; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..456
FT /note="Ammonium transporter Amt2"
FT /id="PRO_0000453009"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT SITE 176
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
FT SITE 343
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
SQ SEQUENCE 456 AA; 46906 MW; B4388737880EC52B CRC64;
MLTALQADLA SVVEGVNLVW VLTVTFLIFF MHAGFAMLEA GQVRAKNVAN QLTKNLLTWS
IGVIVFFLLG AAVSAIVAGL TGGPATTVAD AFMGLYAPDA SATTAWVDWL FGAVFAMTAA
TIVSGAVAGR ARLRAYLTYT ILIAGVIYPV VVGVTWAGGF LNGLGFHDFA GGMIVHGMGG
IAGLTAAWII GPRMNRFNAD GSANVIPGHS ITFAVLGTLI LAFGWYGFNV GTAAAPLAYS
DGGVTLGSFA YVGRVALVTT LGMAAGALGA GGVAFYKTGK VDTLYVANGV LAGLVGITAI
ADDIVWPGAL VVGLLAGAQL PIVFEFVEKR LRIDDVCAVF PVHGSAGVLG TLLYPVFAVP
VWHEGASIVS LAVPQVVGVG VIAVWTFVAT TAIFGGFRAI GQVRVSADHE RQGLDTAEHG
VDTYPEFGSP DADTGIRADG SGIPYGHGFM TTQEDE
