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GUFP_OSTLU
ID   GUFP_OSTLU              Reviewed;         605 AA.
AC   A4RZA6;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Translation factor GUF1 homolog, chloroplastic {ECO:0000255|HAMAP-Rule:MF_03138};
DE            EC=3.6.5.-;
DE   AltName: Full=Elongation factor 4 homolog {ECO:0000255|HAMAP-Rule:MF_03138};
DE            Short=EF-4 {ECO:0000255|HAMAP-Rule:MF_03138};
DE   AltName: Full=GTPase GUF1 homolog {ECO:0000255|HAMAP-Rule:MF_03138};
DE   AltName: Full=Ribosomal back-translocase {ECO:0000255|HAMAP-Rule:MF_03138};
GN   ORFNames=OSTLU_15891;
OS   Ostreococcus lucimarinus (strain CCE9901).
OC   Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC   Bathycoccaceae; Ostreococcus; unclassified Ostreococcus.
OX   NCBI_TaxID=436017;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCE9901;
RX   PubMed=17460045; DOI=10.1073/pnas.0611046104;
RA   Palenik B., Grimwood J., Aerts A., Rouze P., Salamov A., Putnam N.,
RA   Dupont C., Jorgensen R., Derelle E., Rombauts S., Zhou K., Otillar R.,
RA   Merchant S.S., Podell S., Gaasterland T., Napoli C., Gendler K.,
RA   Manuell A., Tai V., Vallon O., Piganeau G., Jancek S., Heijde M.,
RA   Jabbari K., Bowler C., Lohr M., Robbens S., Werner G., Dubchak I.,
RA   Pazour G.J., Ren Q., Paulsen I., Delwiche C., Schmutz J., Rokhsar D.,
RA   Van de Peer Y., Moreau H., Grigoriev I.V.;
RT   "The tiny eukaryote Ostreococcus provides genomic insights into the paradox
RT   of plankton speciation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7705-7710(2007).
CC   -!- FUNCTION: Promotes chloroplast protein synthesis. May act as a fidelity
CC       factor of the translation reaction, by catalyzing a one-codon backward
CC       translocation of tRNAs on improperly translocated ribosomes.
CC       {ECO:0000255|HAMAP-Rule:MF_03138}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_03138};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_03138}.
CC   -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC       transit peptide but none has been predicted. {ECO:0000255|HAMAP-
CC       Rule:MF_03138}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. LepA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03138}.
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DR   EMBL; CP000586; ABO96590.1; -; Genomic_DNA.
DR   RefSeq; XP_001418297.1; XM_001418260.1.
DR   AlphaFoldDB; A4RZA6; -.
DR   SMR; A4RZA6; -.
DR   STRING; 436017.A4RZA6; -.
DR   EnsemblPlants; ABO96590; ABO96590; OSTLU_15891.
DR   GeneID; 5002177; -.
DR   Gramene; ABO96590; ABO96590; OSTLU_15891.
DR   KEGG; olu:OSTLU_15891; -.
DR   eggNOG; KOG0462; Eukaryota.
DR   HOGENOM; CLU_009995_3_3_1; -.
DR   OMA; HIDFNHE; -.
DR   OrthoDB; 165663at2759; -.
DR   Proteomes; UP000001568; Chromosome 6.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:EnsemblPlants.
DR   GO; GO:0045727; P:positive regulation of translation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd03709; lepA_C; 1.
DR   Gene3D; 3.30.70.2570; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_03138; GUFP; 1.
DR   HAMAP; MF_00071; LepA; 1.
DR   InterPro; IPR006297; EF-4.
DR   InterPro; IPR041095; EFG_II.
DR   InterPro; IPR035647; EFG_III/V.
DR   InterPro; IPR000640; EFG_V-like.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027518; GUFP.
DR   InterPro; IPR038363; LepA_C_sf.
DR   InterPro; IPR013842; LepA_CTD.
DR   InterPro; IPR035654; LepA_IV.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43512; PTHR43512; 2.
DR   Pfam; PF00679; EFG_C; 1.
DR   Pfam; PF14492; EFG_III; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF06421; LepA_C; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54980; SSF54980; 2.
DR   TIGRFAMs; TIGR01393; lepA; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Chloroplast; GTP-binding; Hydrolase; Nucleotide-binding; Plastid;
KW   Protein biosynthesis; Reference proteome.
FT   CHAIN           1..605
FT                   /note="Translation factor GUF1 homolog, chloroplastic"
FT                   /id="PRO_0000402917"
FT   DOMAIN          7..189
FT                   /note="tr-type G"
FT   BINDING         16..23
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
FT   BINDING         82..86
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
FT   BINDING         136..139
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
SQ   SEQUENCE   605 AA;  66983 MW;  2CAECE6E5F1E63CF CRC64;
     MASLPARRIR NFSIIAHIDH GKSTLADTLL MRTKTVKERD MVKQVLDSMD LERERGITIK
     LNSARMDYVA SDGELYVLNL IDTPGHVDFS YEVSRSLAAC EGALLVVDSS QGVEAQTVAN
     VYLALENDLE IFTVLNKIDL AGSEPDRVKQ EIEDVLGLDA SDAILASAKA NIGMDDILER
     IVQVVPPPRD TANEPLRALI FDSYFDPYRG VVAVFRVMDG TMKTGDAMKM MATGATFAAD
     EIGIMRPDKV PVKELGPGEV GYIIGGIKSV ADARVGDTIT LSKNPAAEAL PGYSKSVPMV
     FAGIFPTDTD RYDDLRESLQ RLQINDCSLS FEPEQNSAMG TGFRCGFLGL LHMEIIQERL
     EREYDLDLIT TAPSVVYNVY RTDGTMDSIS NPADLCPPEL RERIEEPYCR LDMIAPSDYV
     GTLMELASQR RGEFIDMTYL SETRTSIKYD IPLAEVVTNY FDDMKSRSRG YASMEYAITG
     YRQSDLVRLD VLINQEPADP LAVITHRDRA YSIGRQLVDK LKELIPRQMF RIPIQAAIGN
     KVIAATSISA MRKDVTAKCY GGDISRKKKL LKKQAAGKKR MKQFGKVEVP QDAFLAVLSV
     DGAAD
 
 
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