GUFP_RICCO
ID GUFP_RICCO Reviewed; 693 AA.
AC B9RHQ5;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Translation factor GUF1 homolog, chloroplastic {ECO:0000255|HAMAP-Rule:MF_03138};
DE EC=3.6.5.-;
DE AltName: Full=Elongation factor 4 homolog {ECO:0000255|HAMAP-Rule:MF_03138};
DE Short=EF-4 {ECO:0000255|HAMAP-Rule:MF_03138};
DE AltName: Full=GTPase GUF1 homolog {ECO:0000255|HAMAP-Rule:MF_03138};
DE AltName: Full=Ribosomal back-translocase {ECO:0000255|HAMAP-Rule:MF_03138};
DE Flags: Precursor;
GN ORFNames=RCOM_1767360;
OS Ricinus communis (Castor bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC Ricinus.
OX NCBI_TaxID=3988;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Hale;
RX PubMed=20729833; DOI=10.1038/nbt.1674;
RA Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D.,
RA Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M.,
RA Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J.,
RA Rabinowicz P.D.;
RT "Draft genome sequence of the oilseed species Ricinus communis.";
RL Nat. Biotechnol. 28:951-956(2010).
CC -!- FUNCTION: Promotes chloroplast protein synthesis. May act as a fidelity
CC factor of the translation reaction, by catalyzing a one-codon backward
CC translocation of tRNAs on improperly translocated ribosomes.
CC {ECO:0000255|HAMAP-Rule:MF_03138}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_03138};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_03138}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. LepA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03138}.
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DR EMBL; EQ973780; EEF49142.1; -; Genomic_DNA.
DR AlphaFoldDB; B9RHQ5; -.
DR SMR; B9RHQ5; -.
DR STRING; 3988.XP_002513274.1; -.
DR PRIDE; B9RHQ5; -.
DR eggNOG; KOG0462; Eukaryota.
DR InParanoid; B9RHQ5; -.
DR Proteomes; UP000008311; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0045727; P:positive regulation of translation; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR CDD; cd03709; lepA_C; 1.
DR Gene3D; 3.30.70.2570; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03138; GUFP; 1.
DR HAMAP; MF_00071; LepA; 1.
DR InterPro; IPR006297; EF-4.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027518; GUFP.
DR InterPro; IPR038363; LepA_C_sf.
DR InterPro; IPR013842; LepA_CTD.
DR InterPro; IPR035654; LepA_IV.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43512; PTHR43512; 1.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF06421; LepA_C; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR01393; lepA; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Chloroplast; GTP-binding; Hydrolase; Nucleotide-binding; Plastid;
KW Protein biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
FT CHAIN 52..693
FT /note="Translation factor GUF1 homolog, chloroplastic"
FT /id="PRO_0000402920"
FT DOMAIN 86..267
FT /note="tr-type G"
FT BINDING 95..102
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
FT BINDING 160..164
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
FT BINDING 214..217
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03138"
SQ SEQUENCE 693 AA; 77574 MW; 7E53BA9F810D66C4 CRC64;
MATDLSSSST LLLSRNCKTP PFYHTTNSLS LSKTHHLYAS RNAVVSRLRL LCQTTGSTQS
TPTSGVSDLQ FAAQVGQHRL SKVPISNIRN FCIIAHIDHG KSTLADKLLQ VTGTVQQREM
KEQFLDNMDL ERERGITIKL QAARMRYVYE NKPYCLNLID TPGHVDFSYE VSRSLAACEG
ALLVVDASQG VEAQTLANVY LALENNLEII PVLNKIDLPG SEPDRVIQEI EEVIGLDCSN
AIRCSAKEGI GIIDILNAIV ERIPSPRNTA EMPLRTLIFD SYYDPYRGVI VYFRVLDGSI
KKGDRIYFMA SKKDYFADEI GVLSPNQMQV EELYAGEVGY LSASIRSVAD ARVGDTITHY
NRRAQSSLPG YKEATPMVFC GLFPVDADQF PEVRDALEKL QLNDAALKFE PETSSAMGFG
FRCGFLGLLH MEIVQERLER EYNLSLITTA PSVVYRVNCV DGDTVECSNP SLLPEPGKRR
SIEEPLVKIE MLTPKDYIGP LMELAQDRRG EFKEMRFITN SRASITYELP LAEMVGDFFD
QLKSRSKGYA SMEYTFIGYK ESDLIKLEIQ INGDPVEPLA TIVHKDKAYP VGRALTQKLK
ELIPRQMFKV PIQACIGSKV IASEALPAIR KDVLAKCYGG DISRKKKLLK KQAEGKKRMK
AIGKVDVPQE AFMAVLKLEK KPWSYKYKMS VNC
