AMT2_SCHPO
ID AMT2_SCHPO Reviewed; 512 AA.
AC Q9US00; Q9UU53;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Ammonium transporter 2;
GN Name=amt2; ORFNames=SPAC664.14;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 37-212, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP FUNCTION.
RC STRAIN=FY7406;
RX PubMed=16999738; DOI=10.1111/j.1365-2443.2006.01014.x;
RA Mitsuzawa H.;
RT "Ammonium transporter genes in the fission yeast Schizosaccharomyces pombe:
RT role in ammonium uptake and a morphological transition.";
RL Genes Cells 11:1183-1195(2006).
CC -!- FUNCTION: Transporter for ammonium to use as a nitrogen source.
CC {ECO:0000269|PubMed:16999738}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10759889}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:10759889}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; CU329670; CAB65815.1; -; Genomic_DNA.
DR EMBL; AB027805; BAA87109.1; -; Genomic_DNA.
DR PIR; T50244; T50244.
DR RefSeq; NP_593462.1; NM_001018895.2.
DR AlphaFoldDB; Q9US00; -.
DR SMR; Q9US00; -.
DR BioGRID; 279919; 22.
DR STRING; 4896.SPAC664.14.1; -.
DR MaxQB; Q9US00; -.
DR PaxDb; Q9US00; -.
DR EnsemblFungi; SPAC664.14.1; SPAC664.14.1:pep; SPAC664.14.
DR GeneID; 2543501; -.
DR KEGG; spo:SPAC664.14; -.
DR PomBase; SPAC664.14; amt2.
DR VEuPathDB; FungiDB:SPAC664.14; -.
DR eggNOG; KOG0682; Eukaryota.
DR HOGENOM; CLU_000445_33_0_1; -.
DR InParanoid; Q9US00; -.
DR OMA; HFAFIRT; -.
DR PhylomeDB; Q9US00; -.
DR PRO; PR:Q9US00; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:PomBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0015200; F:methylammonium transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:PomBase.
DR GO; GO:0072489; P:methylammonium transmembrane transport; IMP:PomBase.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
PE 3: Inferred from homology;
KW Ammonia transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..512
FT /note="Ammonium transporter 2"
FT /id="PRO_0000278388"
FT TOPO_DOM 1..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..137
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..267
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..354
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 376..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..512
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 512 AA; 55570 MW; D07E77A48947A94D CRC64;
MSSVNSIPTA TSTVYISVLP ATATPSGGSG GNVLHEDLNK FYDYGNTSWI LACTPLCLIM
VPGVAFFYSG LARRKNTLAL IMLSMLGLCV SFFQWYFWGY SLAFSQTGTS GYIGNLRHFA
FIRTLADYSP GSNNIPELVF ANFQGMFAAI TVALFTGAAA ERGRIGPMLI ITFVWLTVVY
CPIACWIWNP NGWAFKFGVY DFAGGGPVEV GSGFAALAYT VCLGRRSKFV EEQFRPHSVL
NVVLGTSLLW FGWLGFNGGS AYGSNLRAAM AITNTNLAGA VAGLVWVIYD YIFRTRKWST
IGFCSGVVAG LVAATPCAGF VSPHASLAIG AITGLCCNWA IKLKSHMRID DAMDIFAIHG
VAGFVGTFLN GLFAVDYIAA MDGIYVGENK IRGGWFDHHW RQLGLQMAYI CAVGAYDFVV
TFIILFITDK IPYLQLRVSP DAEEIGVDAD QIGEYAFDYI EERREYKHWK ISPAGVPEEI
IISNGVAQPT GNVAAPGKIL ESTNPLELGL TI
