GUN10_ORYSJ
ID GUN10_ORYSJ Reviewed; 620 AA.
AC Q84R49; A0A0N7KI04; Q0DNT2;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Endoglucanase 10;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 10;
DE AltName: Full=OsGLU2;
GN Name=GLU2; OrderedLocusNames=Os03g0736300, LOC_Os03g52630;
GN ORFNames=OsJ_12485, OSJNBb0016H12.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14530400; DOI=10.1073/pnas.1434476100;
RA Ilic K., SanMiguel P.J., Bennetzen J.L.;
RT "A complex history of rearrangement in an orthologous region of the maize,
RT sorghum, and rice genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:12265-12270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA Chen S.-Y.;
RT "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT affects plant internode elongation.";
RL Plant Mol. Biol. 60:137-151(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16463105}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AY387483; AAR07086.1; -; Genomic_DNA.
DR EMBL; AC118133; AAP03405.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98746.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13106.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86265.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE59884.1; -; Genomic_DNA.
DR EMBL; AK070408; BAG91933.1; -; mRNA.
DR RefSeq; XP_015630455.1; XM_015774969.1.
DR AlphaFoldDB; Q84R49; -.
DR SMR; Q84R49; -.
DR STRING; 4530.OS03T0736300-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q84R49; -.
DR PRIDE; Q84R49; -.
DR EnsemblPlants; Os03t0736300-01; Os03t0736300-01; Os03g0736300.
DR GeneID; 4334029; -.
DR Gramene; Os03t0736300-01; Os03t0736300-01; Os03g0736300.
DR KEGG; osa:4334029; -.
DR eggNOG; ENOG502SJG7; Eukaryota.
DR HOGENOM; CLU_008926_1_3_1; -.
DR InParanoid; Q84R49; -.
DR OMA; FIMMIVK; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q84R49; baseline and differential.
DR Genevisible; Q84R49; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase; Membrane;
KW Polysaccharide degradation; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..620
FT /note="Endoglucanase 10"
FT /id="PRO_0000249287"
FT TRANSMEM 72..92
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 165
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 513
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 561
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 570
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 216
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 314
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 323
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 408
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 425
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 567
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 620 AA; 69067 MW; 2B5721EBD2ED7D52 CRC64;
MFGRDPWGGP LEISNADSAT DDDRSRDLDR GALMRQLDET QQSWLLAGPG DQAGKKKKKY
VDLGCMVLDR KIFMWTVGTI LGVGLFIGFV MMIVKLVPHK RPPPPPPDQY TQALHKALMF
FNAQRSGPLP KHNGVSWRGN SCMKDGLSDS TVRKSLVGGF YDAGDAIKFN YPMAWSMTML
SWSVIEYKAK YEAIGELDHV KELIKWGTDY LLKTFNSSAD TIDRIVAQVG VGDTSKGGAQ
PNDHYCWMRP EDIDYPRPVT ECHSCSDLAS EMAAALAAAS IVFKDSKTYS DKLVRGAKAL
YKFGRLQRGR YSPNGSDQAI FYNSTSYWDE FVWGGAWMYF ATGNNTYLSV ATAPGMAKHA
GAYWLDSPNY GVFTWDDKLP GAQVLLSRLR LFLSPGYPYE EILRTFHNQT DNVMCSYLPM
YNSFNFTKGG MIQLNHGRPQ PLQYVVNAAF LASLYSDYLD AADTPGWYCG PTFYTTEVLR
KFARSQLDYV LGKNPLKMSY VVGFGNKYPK RAHHRGASIP HNGVKYGCKG GFKWRETKKP
NPNILIGALV AGPDRHDGFK DVRTNYNYTE PTLAANAGLV AALISLTNIH VKSGIDKNTI
FSAVPPMFPT PPPPPSAWKP
