GUN11_ARATH
ID GUN11_ARATH Reviewed; 525 AA.
AC O48766;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Endoglucanase 11;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 11;
DE Flags: Precursor;
GN OrderedLocusNames=At2g32990; ORFNames=T21L14.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AC003033; AAB91971.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08771.1; -; Genomic_DNA.
DR EMBL; AY062439; AAL32517.1; -; mRNA.
DR EMBL; BT008885; AAP68324.1; -; mRNA.
DR PIR; T01108; T01108.
DR RefSeq; NP_180858.1; NM_128859.3.
DR AlphaFoldDB; O48766; -.
DR SMR; O48766; -.
DR STRING; 3702.AT2G32990.1; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; O48766; -.
DR PRIDE; O48766; -.
DR ProteomicsDB; 230177; -.
DR EnsemblPlants; AT2G32990.1; AT2G32990.1; AT2G32990.
DR GeneID; 817861; -.
DR Gramene; AT2G32990.1; AT2G32990.1; AT2G32990.
DR KEGG; ath:AT2G32990; -.
DR Araport; AT2G32990; -.
DR TAIR; locus:2059375; AT2G32990.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; O48766; -.
DR OMA; CQDNFVD; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; O48766; -.
DR BioCyc; ARA:AT2G32990-MON; -.
DR PRO; PR:O48766; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48766; baseline and differential.
DR Genevisible; O48766; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..525
FT /note="Endoglucanase 11"
FT /id="PRO_0000249263"
FT ACT_SITE 92
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 427
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 479
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 488
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT CARBOHYD 337
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 525 AA; 59087 MW; 6E3C3A23165EE44F CRC64;
MTVMNHRHKQ CHFLLLLLIT VFSAALDGVS SETDVGGFDY GEALSKSLLY FEAQRSGRLP
YNQRVTWRDH SGLTDGLEQG VDLVGGYHDA GDHVKFGLPM AFTVTMLSWS VIEYGDSLAS
TGELSHALEA IKWGTDYFIK AHTSPNVLWA EVGDGDTDHY CWQRPEDMTT SRRAFKIDEN
NPGSDIAGET AAAMAAASIV FRSTNPHYSH LLLHHAQQLF EFGDKYRGKY DESLKVVKSY
YASVSGYMDE LLWGATWLYR ATDNEHYMSY VVDMAHQLGG LSWAMSEFSW DVKFAGVQLL
ASMLLKEEKH KQHSKVLQQY KSKADHYLCS ILNKNINGTN VQRTPAGLLY VRQWNNMQYV
STASFLLTVY SDHLRKSNTD LECHEGTVTP DEMLGFAKSQ IDYILGSNPM ETSYLVGYGP
KYPIRVHHRG ASIASFKEHK GFIGCTQGYD NWYGRSEPNP SVLVGALVGG PDHQDDFDDR
RGNYVQTEAC TYNTAPLVGV FARLIELEEQ KLEEEDVSLV ATYKR
