GUN12_ORYSJ
ID GUN12_ORYSJ Reviewed; 623 AA.
AC Q7XUK4; Q0JC19;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Endoglucanase 12;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 12;
DE AltName: Full=OsGLU3;
GN Name=GLU3; OrderedLocusNames=Os04g0497200, LOC_Os04g41970;
GN ORFNames=OSJNBa0067K08.14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA Chen S.-Y.;
RT "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT affects plant internode elongation.";
RL Plant Mol. Biol. 60:137-151(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16463105}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15118.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL606627; CAD41250.2; -; Genomic_DNA.
DR EMBL; AP008210; BAF15118.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; BAS89897.1; -; Genomic_DNA.
DR EMBL; AK243593; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015636553.1; XM_015781067.1.
DR AlphaFoldDB; Q7XUK4; -.
DR SMR; Q7XUK4; -.
DR STRING; 4530.OS04T0497200-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q7XUK4; -.
DR PRIDE; Q7XUK4; -.
DR EnsemblPlants; Os04t0497200-01; Os04t0497200-01; Os04g0497200.
DR GeneID; 4336284; -.
DR Gramene; Os04t0497200-01; Os04t0497200-01; Os04g0497200.
DR KEGG; osa:4336284; -.
DR eggNOG; ENOG502QSIM; Eukaryota.
DR HOGENOM; CLU_008926_1_3_1; -.
DR InParanoid; Q7XUK4; -.
DR OMA; TMCSYLH; -.
DR OrthoDB; 1195424at2759; -.
DR PlantReactome; R-OSA-9030654; Primary root development.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; Q7XUK4; baseline and differential.
DR Genevisible; Q7XUK4; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase; Membrane;
KW Polysaccharide degradation; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..623
FT /note="Endoglucanase 12"
FT /id="PRO_0000249289"
FT TOPO_DOM 1..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..623
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT ACT_SITE 166
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 513
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 561
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 570
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 217
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 236
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 324
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 345
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 408
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 425
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 623 AA; 69243 MW; BAEF21481AEF18B2 CRC64;
MYSANHWGGS FEIAADGAAE DDHSRNMDLD RGALSARQHQ LDETQQSWLL GPPEAKKKDK
YVDLGCVVVK RKLLWWVLWT LLAAFILIGL PVIIAKSIPK KKPHAPPPDQ YTDALHKALL
FFNAQKSGRL PKNNGIKWRG NSGLSDGSDL TDVKGGLVGG YYDAGDNIKF HFPLAFSMTM
LSWSVIEYSA KYKAVGEYDH VRELIKWGTD YLLLTFNSSA STIDKVYSQV GIAKINGTQP
DDHYCWNRPE DMAYPRPVQT AGSAPDLGGE MAAALAAASI VFRDNAAYSK KLVNGAAAVY
KFARSSGRRT PYSRGNQYIE YYYNSTSYWD EYMWSAAWMY YATGNNTYIT FATDPRLPKN
AKAFYSILDF SVFSWDNKLP GAELLLSRLR MFLNPGYPYE ESLIGYHNTT SMNMCTYFPR
FGAFNFTKGG LAQFNHGKGQ PLQYTVANSF LAALYADYME SVNVPGWYCG PYFMTVDDLR
SFARSQVNYI LGDNPKKMSY VVGYGKKYPR RLHHRGASTP HNGIKYSCTG GYKWRDTKGA
DPNVLVGAMV GGPDKNDQFK DARLTYAQNE PTLVGNAGLV AALVALTNSG RGAGVTAVDK
NTMFSAVPPM FPATPPPPSK WKP
