GUN13_ORYSI
ID GUN13_ORYSI Reviewed; 625 AA.
AC A2XYW8; Q259H9; Q7XQ92;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Endoglucanase 13;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 13;
DE AltName: Full=OsGLU6;
DE Flags: Precursor;
GN Name=GLU6; ORFNames=H0103C06.3, H0403D02.19, OsI_017261;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Guang-Lu-Ai No.4;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AL732344; CAH68191.1; -; Genomic_DNA.
DR EMBL; AL732351; CAJ86099.1; -; Genomic_DNA.
DR EMBL; CM000129; EAY96028.1; -; Genomic_DNA.
DR AlphaFoldDB; A2XYW8; -.
DR SMR; A2XYW8; -.
DR STRING; 39946.A2XYW8; -.
DR CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR HOGENOM; CLU_008926_1_4_1; -.
DR Proteomes; UP000007015; Chromosome 4.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Glycosidase;
KW Hydrolase; Polysaccharide degradation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..625
FT /note="Endoglucanase 13"
FT /id="PRO_0000295017"
FT REGION 509..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 91
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 427
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 479
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 488
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 440
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 230
FT /note="D -> H (in Ref. 2; EAY96028)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 625 AA; 68381 MW; D220BF0D009441F1 CRC64;
MAATMNKTPA TTFLLIPAAA SLVLLLAAAA SVEASAFDYA GAFDKCLLFF EAQRSGKLPD
DRLVRWRGDS ALTDGFSQGV DLVGGYYDSG DHVKFGLPMA YAVTMLSWGV VEFEKEMVDG
NKLHRVLDAI RWGTNYFVKA HTQHNALWVQ VGDGDSDHLC WERAEDMSTP RTAFKIDINN
PGSEVAGETA AALAAAAKAF KPYDRMYSDL LLLHSKQLFT FADTFRGKYD DSLQSAKKFY
PSASGYQDEL LWAAAWLYEA TGDEQYLRYV SQNAEAFGGT GWAVTEFSWD NKYAGLQVLL
SKVLFEQGGS AAGYADTLKQ YQAKAEFFLC ACLQKNNGHN VKMTPGGLMY VSDWSNMQYV
SSSAFLLTVY ADYLAESRGT LRCPDGEVKP AEILLFARSQ VDYVLGKNPK GMSYMVGYGS
YYPTHVHHRG ASIPSIYAMN ATVGCMEGFD KYYNSKNADP NVLHGALVGG PDANDAYDDD
RCNYQHAEPT LAGNAPMSGV FARLAASPAD NTPEYTPAPN APSPSNGGSP LEFVHTVTNT
WKANGVDYYR HVVTAKNTCG HAITYLKLQI KELSGEIYGV SRTNAKDMYE FPSWMTRLDA
GAQLTIVYIQ GGPAAKIAVV EYKTA
