ID   GUN13_ORYSJ             Reviewed;         625 AA.
AC   Q0J930; B7F5B2; Q259H9; Q7XQ92;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Endoglucanase 13;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 13;
DE   AltName: Full=OsGLU6;
DE   Flags: Precursor;
GN   Name=GLU6; OrderedLocusNames=Os04g0674800, LOC_Os04g57860;
GN   ORFNames=OSJNBa0018M05.16;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   TISSUE SPECIFICITY.
RX   PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA   Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA   Chen S.-Y.;
RT   "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT   affects plant internode elongation.";
RL   Plant Mol. Biol. 60:137-151(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots and flowers.
CC       {ECO:0000269|PubMed:16463105}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AL606457; CAE03241.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF16157.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS91608.1; -; Genomic_DNA.
DR   EMBL; AK119913; BAG99809.1; -; mRNA.
DR   RefSeq; XP_015634039.1; XM_015778553.1.
DR   AlphaFoldDB; Q0J930; -.
DR   SMR; Q0J930; -.
DR   STRING; 4530.OS04T0674800-01; -.
DR   CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; Q0J930; -.
DR   PRIDE; Q0J930; -.
DR   EnsemblPlants; Os04t0674800-01; Os04t0674800-01; Os04g0674800.
DR   GeneID; 4337384; -.
DR   Gramene; Os04t0674800-01; Os04t0674800-01; Os04g0674800.
DR   KEGG; osa:4337384; -.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; Q0J930; -.
DR   OMA; TNAKDMY; -.
DR   OrthoDB; 1195424at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q0J930; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR019028; CBM_49.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF09478; CBM49; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SMART; SM01063; CBM49; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..625
FT                   /note="Endoglucanase 13"
FT                   /id="PRO_0000249290"
FT   REGION          509..530
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        91
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        427
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        479
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        488
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        440
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   625 AA;  68454 MW;  ED817D88518F62B5 CRC64;
     MAATMNKTPA TTFLLIPAAA SLVLLLAAAA SVEASAFDYA GAFDKCLLFF EAQRSGKLPD
     DRLVRWRGDS ALTDGFSQGV DLVGGYYDSG DHVKFGLPMA YAVTMLSWGV VEFEKEMVDG
     NKLHRVLDAI RWGTNYFVKA HTQHNALWVQ VGDGDSDHLC WERAEDMSTP RTAFKIDINN
     PGSEVAGETA AALAAAAKAF KPYDRMYSDL LLLHSKQLFT FADTFRGKYD DSLQSAKKFY
     PSASGYQDEL LWAAAWLYEA TGDEQYLRYV SQNAEAFGGT GWAVTEFSWD NKYAGLQVLL
     SKVLFEQGGS AAGYADTLKQ YQAKAEFFLC ACLQKNNGHN VKMTPGGLMY VSDWSNMQYV
     SSSAFLLTVY ADYLAESRGT LRCPDGEVKP AEILRFARSQ VDYVLGKNPK GMSYMVGYGS
     YYPTHVHHRG ASIPSIYAMN ATVGCMESFD KYYNSKNADP NVLHGALVGG PDANDAYDDD
     RCNYQHAEPT LAGNAPMSGV FARLAASPAD NTPEYTPAPN APSPSNGGSP LEFVHTVTNT
     WKANGVDYYR HVVTAKNTCG HAITYLKLQI KELSGEIYGV SRTNAKDMYE FPSWMTRLDA
     GAQLTIVYIQ GGPAAKIAVV EYKTA