GUN14_ARATH
ID GUN14_ARATH Reviewed; 491 AA.
AC Q8S8Q4; O64891;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Endoglucanase 14;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 14;
DE Flags: Precursor;
GN OrderedLocusNames=At2g44560; ORFNames=F16B22.5, F4I1.39;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM14961.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003672; AAC27458.1; -; Genomic_DNA.
DR EMBL; AC004521; AAM14961.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10438.1; -; Genomic_DNA.
DR EMBL; AK175889; BAD43652.1; -; mRNA.
DR PIR; T01583; T01583.
DR RefSeq; NP_181984.1; NM_130020.3.
DR AlphaFoldDB; Q8S8Q4; -.
DR SMR; Q8S8Q4; -.
DR STRING; 3702.AT2G44560.1; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q8S8Q4; -.
DR PRIDE; Q8S8Q4; -.
DR ProteomicsDB; 247266; -.
DR EnsemblPlants; AT2G44560.1; AT2G44560.1; AT2G44560.
DR GeneID; 819064; -.
DR Gramene; AT2G44560.1; AT2G44560.1; AT2G44560.
DR KEGG; ath:AT2G44560; -.
DR Araport; AT2G44560; -.
DR TAIR; locus:2042451; AT2G44560.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q8S8Q4; -.
DR OMA; EEYICSC; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; Q8S8Q4; -.
DR BioCyc; ARA:AT2G44560-MON; -.
DR PRO; PR:Q8S8Q4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8Q4; baseline and differential.
DR Genevisible; Q8S8Q4; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..491
FT /note="Endoglucanase 14"
FT /id="PRO_0000249266"
FT ACT_SITE 86
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 413
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 474
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT CARBOHYD 397
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 491 AA; 53198 MW; 7F9250C761AA8484 CRC64;
MSQLKIGSSQ CLWTSICIVL FVLSMARGAV SRNYGDALTK SLLYFEAQRS GKLPSNQRVT
WRGDSALRDG SDAHVDLTGG YYDAGDNMKF GFPLAFFTTM LAWSNIEMAT QLKAHQEQEN
ALAALKWATD FLIKAHPEPN VLYGQVGDGN SDHECWMRPE DMTTPRPSFR IDAQHPGSDL
AGETAAAMAA ASIAFAPSDE AYAQILIGHA KELFEFAKAY PGIYQNSITN AGGFYASSGY
EDELLWAAAW LHRATNDQIY LDYLTQASGT GGPRTAFSWD DKFVGAQVLV AKLALEGKVE
SNGKIAEYKS MAEQFICNCA QKGSNNVKKT PGGLLYFLPW NNLQYTTAAT FVLSAYSKYL
TDAKASIQCP NGALQASDLL DLARSQVDYI LGSNPQNMSY MVGVGTNYPK KPHHRAASIV
SITKDKTPVT CSEGFDAWFN NPAPNPNVLM GAVVGGPNDN DVYGDERTDY QHAEPAPATA
APFVGVLAAV A
