GUN15_ORYSJ
ID GUN15_ORYSJ Reviewed; 629 AA.
AC Q6L4I2; A0A0P0WJ95; Q0DJY9;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Endoglucanase 15;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 15;
DE AltName: Full=OsCel9C;
DE Flags: Precursor;
GN OrderedLocusNames=Os05g0212300, LOC_Os05g12150; ORFNames=OSJNBa0074P11.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC135914; AAT44235.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16834.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92803.1; -; Genomic_DNA.
DR EMBL; AK063458; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q6L4I2; -.
DR SMR; Q6L4I2; -.
DR STRING; 4530.OS05T0212300-01; -.
DR CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6L4I2; -.
DR PRIDE; Q6L4I2; -.
DR EnsemblPlants; Os05t0212300-01; Os05t0212300-01; Os05g0212300.
DR Gramene; Os05t0212300-01; Os05t0212300-01; Os05g0212300.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q6L4I2; -.
DR OMA; ASMIFRT; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6L4I2; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..629
FT /note="Endoglucanase 15"
FT /id="PRO_0000249292"
FT ACT_SITE 87
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 421
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 473
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 482
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 540
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 561
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 284
FT /note="G -> E (in Ref. 5; AK063458)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 629 AA; 68664 MW; 11FBA6DCF13F66DE CRC64;
MAKNGGAHGA ATLFGLLALA SMVKLGFVAG GGHDYAMALR KSILYFQAQR SGVLPPNQRV
SWRASSGLFD GKANGVDLVG GYYDAGDNVK FGLPMAFTVT MMSWSILEYG KQMAAAGELR
NAMDAVKWGT DYFIKAHPEP DVLYGEVGDG DTDHSCWQRP EDMTTSRQAF RVDPQHPGSD
LAAETAAAMA AASIVFRGTY PGYANLLLVH SKQLFEFADK YRGKYDASIT VARNYYGSFS
GYGDELLWAA AWLFEATEDR SYLEYLAGNG EALGGTGWSI NQFGWDVKYP GVQVLAAKFL
LQGRAGDHAA ALQRYRQNAE FFVCSCVGKG AVNVARTPGG MMYHQRWNNL QFVTSASFLL
TVYADFAAIS GRGAVHCPAG AAQPFDILKF VKSQVNYILG DNPRGTSYMV GYGASYPRQV
HHRGASIVSI KRDPSFVSCQ EGYSSWYGRE AGNPNLLDGA VVGGPDEYDD FADERDNYEQ
TEAATYNNAP LLGVLARLAA SCGGLKEEEY EQETATPVVN RTSSSSSLPA TATAIGIEQN
VTGTWARRRR TYYRYAVTVT NRSRGKTVRE LHLGVSGLRG RLWGLEEARY GYVPPRWLPA
LRPGRSLRFV YVQPAPAPAN IWVTGYKLV
