GUN16_ARATH
ID GUN16_ARATH Reviewed; 486 AA.
AC Q8VYG3; Q9LZG2;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Endoglucanase 16;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 16;
DE Flags: Precursor;
GN OrderedLocusNames=At3g43860; ORFNames=T28A8.150;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB83158.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL162691; CAB83158.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77836.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65868.1; -; Genomic_DNA.
DR EMBL; AY072099; AAL59921.1; -; mRNA.
DR PIR; T47422; T47422.
DR RefSeq; NP_001327805.1; NM_001339113.1.
DR RefSeq; NP_189972.2; NM_114254.3.
DR AlphaFoldDB; Q8VYG3; -.
DR SMR; Q8VYG3; -.
DR STRING; 3702.AT3G43860.1; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q8VYG3; -.
DR PRIDE; Q8VYG3; -.
DR ProteomicsDB; 247315; -.
DR EnsemblPlants; AT3G43860.1; AT3G43860.1; AT3G43860.
DR EnsemblPlants; AT3G43860.2; AT3G43860.2; AT3G43860.
DR GeneID; 823499; -.
DR Gramene; AT3G43860.1; AT3G43860.1; AT3G43860.
DR Gramene; AT3G43860.2; AT3G43860.2; AT3G43860.
DR KEGG; ath:AT3G43860; -.
DR Araport; AT3G43860; -.
DR TAIR; locus:2101099; AT3G43860.
DR eggNOG; ENOG502QQ06; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q8VYG3; -.
DR OMA; PSWRGDS; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; Q8VYG3; -.
DR BioCyc; ARA:AT3G43860-MON; -.
DR PRO; PR:Q8VYG3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VYG3; baseline and differential.
DR Genevisible; Q8VYG3; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..486
FT /note="Endoglucanase 16"
FT /id="PRO_0000249268"
FT ACT_SITE 87
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 407
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 458
FT /evidence="ECO:0000250"
FT ACT_SITE 467
FT /evidence="ECO:0000250"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 486 AA; 54120 MW; 0511043BBFCCD036 CRC64;
MANYKGRGNV MIRSMLLGLY GIINIVCVNG TFINYKDALT KSLIFLEAQR SGKLPPNNRV
PWRGDSALDD GKLVNVDLSG GYYDAGDNVK YGLPMAFTIT TLAWSTITYE KELRATGELE
NARAAIRWGT DYFLKCASRK NRLYVQVGDP NADHQCWARP ENMKTPRTVL EISDKVPGTE
IAAEAAAAFA ASSIVFRHVD HKYARRLLNK AKLLFKLAKS HKGTYDGECP FYCSNSGYND
ELIWAATWLY KATRNHLYLS YLKFEAISAY VAEFSWDLKY AGAQILITKL IFEGHKGLDL
YKQQADSFVC SNLPGSPYHQ VFTTPGGMIH LRDGANSQYV TATAFLFSAY ADILQKHNQK
ISCGSHQFDS THLMAFAKKQ IDYILGHNPQ GRSYMVGFGP NPPKQAHHRG ASVPMHEANA
PLSCPLSFVK WYNKNVPNAN ELTGAILGGP DRQDKFQDLR WTSVYTEPCT YINSIAVGVL
AKLAAA
