GUN16_ORYSJ
ID GUN16_ORYSJ Reviewed; 538 AA.
AC Q654U4; Q0DD73;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Endoglucanase 16;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 16;
DE Flags: Precursor;
GN OrderedLocusNames=Os06g0247900, LOC_Os06g13830; ORFNames=OJ1136_C11.27;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q654U4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q654U4-2; Sequence=VSP_020389;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD45673.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF19200.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004027; BAD45673.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008212; BAF19200.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK065325; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015641192.1; XM_015785706.1. [Q654U4-1]
DR AlphaFoldDB; Q654U4; -.
DR SMR; Q654U4; -.
DR STRING; 4530.OS06T0247900-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q654U4; -.
DR PRIDE; Q654U4; -.
DR GeneID; 4340645; -.
DR KEGG; osa:4340645; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_0_1; -.
DR InParanoid; Q654U4; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q654U4; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carbohydrate metabolism;
KW Cell wall biogenesis/degradation; Cellulose degradation; Glycosidase;
KW Hydrolase; Polysaccharide degradation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..538
FT /note="Endoglucanase 16"
FT /id="PRO_0000249293"
FT REGION 513..538
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 83
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 431
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 483
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 492
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT VAR_SEQ 1..158
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_020389"
SQ SEQUENCE 538 AA; 58893 MW; 94ED0101E3408692 CRC64;
MRWRRVGDVV AVALLLGAAA AAAAAAARHD YEEALRKSLL YFEAQRSGRL PHGQRVAWRD
HSGLTDGLEQ GVDLVGGYYD AGDHVKFGLP MAFTVTMLSW SLLEYGADVA AAGELAHALD
AIKWGTDYFI KAHTKPHELW AEVGDGDTDH YCWQRPEDMT TSRQAYKVDR RRPGSDVAGE
TAAAMAAASI VFRQSNPHYS HLLLHHAQQL FEFADTYRGK YDSSIAEVKS YYASVSGYHD
ELLWAALWLH RATGRAAYLD YAVDNADEFG GTGWAITEFS WDVKYAGVQI LAARLLMRGE
HEERHRGTLE RYREKAEHYV CACMGRNAAG GADANVERSP GGMLYVRQWN NMQYVTNAAF
LLSAYSDYLA GAGDGDGDGG GGVATCVGGG GAGAGEVFAA AREQVDYVLG SNPRGMSYLV
GYGERFPARV HHRAASIVPY KDSKEFIGCA QGFDDWFGRR GANPNVVVGA IVGGPDRRDR
FRDDRENYMQ TEACTYNTAP MVGMFAMLNR LSRQESPSTT TTTTATTSSP EMGLSVNR
