GUN17_ORYSJ
ID GUN17_ORYSJ Reviewed; 497 AA.
AC Q652F9; A0A0P0WUX1; Q0DD41;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Endoglucanase 17;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 17;
DE AltName: Full=OsGLU13;
DE Flags: Precursor;
GN Name=GLU13; OrderedLocusNames=Os06g0256900, LOC_Os06g14540;
GN ORFNames=OsJ_020012, P0624H09.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP005619; BAD46308.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19232.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS97102.1; -; Genomic_DNA.
DR EMBL; CM000143; EAZ36529.1; -; Genomic_DNA.
DR EMBL; AK060686; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015641944.1; XM_015786458.1.
DR AlphaFoldDB; Q652F9; -.
DR SMR; Q652F9; -.
DR STRING; 4530.OS06T0256900-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q652F9; -.
DR PRIDE; Q652F9; -.
DR EnsemblPlants; Os06t0256900-01; Os06t0256900-01; Os06g0256900.
DR GeneID; 4340679; -.
DR Gramene; Os06t0256900-01; Os06t0256900-01; Os06g0256900.
DR KEGG; osa:4340679; -.
DR eggNOG; ENOG502QR9R; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q652F9; -.
DR OMA; GDAFHDH; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q652F9; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..497
FT /note="Endoglucanase 17"
FT /id="PRO_0000249294"
FT ACT_SITE 77
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 406
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 458
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 467
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 451
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 331
FT /note="V -> A (in Ref. 5; AK060686)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 54384 MW; 7AA1465C0A72B8D3 CRC64;
MAAAGGAVLL LVLATATSVT GQHDYSDALH KSILFFEGQR SGRLPPDQRL RWRRDSALND
GATAGVDLTG GYYDAGDNVK FGFPMAFTAT LMSWGLIDFG RSFGAHAAEA REAVRWATDY
LMKATATPNT VYVQVGDAFR DHSCWERPED MDTPRTVYKV DPSHPGSDVA AETAAALAAA
SIVFRDADPD YSNRLLDRAI QVFEFADKYR GPYSSSLHAA VCPCYCDYSG YKDELLWGAA
WLHKASRRRE YRDYIKRNEV VLGASEAINE FGWDNKHAGI NVLISKEVLM GKDEYFQSFR
VNADNFICTL LPGISNHPQI QYSPGGLLFK VGNSNMQHVT SLSFLLLAYS NYLSHANVRV
PCGTSSASPV QLRRVAKRQV DYILGDNPLR MSYMVGYGSR YPLRIHHRGS SLPSVAAHPA
QIGCKAGATY YASAAPNPNL LVGAVVGGPS NTSDAFPDAR AVFQQSEPTT YINAPLLGLL
AYFSAHPNLA QSDLLYD