ID   GUN19_ORYSJ             Reviewed;         523 AA.
AC   Q6YXT7; A0A0P0XB73; Q0J8F3;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Endoglucanase 19;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 19;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os08g0114200, LOC_Os08g02220; ORFNames=P0427G12.14;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AP005657; BAD10555.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF22762.1; -; Genomic_DNA.
DR   EMBL; AP014964; BAT03541.1; -; Genomic_DNA.
DR   EMBL; AK106851; BAG97853.1; -; mRNA.
DR   RefSeq; XP_015649019.1; XM_015793533.1.
DR   AlphaFoldDB; Q6YXT7; -.
DR   SMR; Q6YXT7; -.
DR   STRING; 4530.OS08T0114200-01; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; Q6YXT7; -.
DR   PRIDE; Q6YXT7; -.
DR   EnsemblPlants; Os08t0114200-01; Os08t0114200-01; Os08g0114200.
DR   GeneID; 4344508; -.
DR   Gramene; Os08t0114200-01; Os08t0114200-01; Os08g0114200.
DR   KEGG; osa:4344508; -.
DR   eggNOG; ENOG502QPI6; Eukaryota.
DR   HOGENOM; CLU_008926_1_2_1; -.
DR   InParanoid; Q6YXT7; -.
DR   OMA; WGASWIY; -.
DR   OrthoDB; 1195424at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   Genevisible; Q6YXT7; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..52
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..523
FT                   /note="Endoglucanase 19"
FT                   /id="PRO_0000249296"
FT   ACT_SITE        107
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        442
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        493
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        502
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        279
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   523 AA;  56214 MW;  F047BC4FC913A022 CRC64;
     MCSWSLSSHT LTSPVRQAAM EPKSSSCGGA GIRLRLLVVL HLLLLVPSSA MAFNYADALA
     KSIIFFEGQR SGKLPPGNRM PWRADSGLTD GAQYNVDLVG GYYDAGDNVK FGLPMAFSTT
     MLAWSVLDFG KFMGAELPNA RAAVRWGADY LLKAATATPG ALYVQVADPN QDHRCWERPE
     DMDTPRSVYR VTADKPGSDV AGETAAALAA SSMVFRRADP AYSARLLHAA TQVFDFADRH
     RGSYSDSLAS SVCPFYCSYS GYHDELLWGA SWLHRASRNA SFMSYVEANG MQLGAGDDDY
     SFSWDDKRVG TKVLLAKGFL RNRLHGLELY KAHSDSYICS LVPGTASFQS RYTPGGLLYR
     EGSSNMQYVT TATFLMLAYA KYLRSSGATA SCGDGGGGAR GEVSAAELVA VAKRQVDYIL
     GKNPAGMSYM VGFGCRYPRR AHHRGASMPS VRAHPGRISC DAGFGYLHSG EPNPNVLVGA
     VVGGPDSRDA FADDRGNFAQ SEPATYINAP LVGALAYFAG TTK