GUN1_ARATH
ID GUN1_ARATH Reviewed; 501 AA.
AC Q9SRX3; O64949;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Endoglucanase 1;
DE EC=3.2.1.4;
DE AltName: Full=Cellulase 2;
DE Short=AtCEL2;
DE AltName: Full=Endo-1,4-beta glucanase 1;
DE Flags: Precursor;
GN Name=CEL2; OrderedLocusNames=At1g02800; ORFNames=F22D16.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10074717; DOI=10.1046/j.1365-313x.1999.00359.x;
RA Yung M.-H., Schaffer R., Putterill J.;
RT "Identification of genes expressed during early Arabidopsis carpel
RT development by mRNA differential display: characterisation of ATCEL2, a
RT novel endo-1,4-beta-D-glucanase gene.";
RL Plant J. 17:203-208(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing septum and ovule
CC primordia during the early stages of flower development.
CC {ECO:0000269|PubMed:10074717}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AF034573; AAC16418.1; -; mRNA.
DR EMBL; AC009525; AAF02887.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27471.1; -; Genomic_DNA.
DR EMBL; AK228979; BAF00867.1; -; mRNA.
DR PIR; A86158; A86158.
DR PIR; T52135; T52135.
DR RefSeq; NP_171779.1; NM_100159.3.
DR AlphaFoldDB; Q9SRX3; -.
DR SMR; Q9SRX3; -.
DR STRING; 3702.AT1G02800.1; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR MetOSite; Q9SRX3; -.
DR PaxDb; Q9SRX3; -.
DR PRIDE; Q9SRX3; -.
DR ProteomicsDB; 247240; -.
DR EnsemblPlants; AT1G02800.1; AT1G02800.1; AT1G02800.
DR GeneID; 839385; -.
DR Gramene; AT1G02800.1; AT1G02800.1; AT1G02800.
DR KEGG; ath:AT1G02800; -.
DR Araport; AT1G02800; -.
DR TAIR; locus:2024670; AT1G02800.
DR eggNOG; ENOG502QPI6; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q9SRX3; -.
DR OMA; MTWRSDS; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; Q9SRX3; -.
DR BioCyc; ARA:AT1G02800-MON; -.
DR PRO; PR:Q9SRX3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SRX3; baseline and differential.
DR Genevisible; Q9SRX3; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; TAS:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0007389; P:pattern specification process; TAS:TAIR.
DR GO; GO:0009624; P:response to nematode; IEP:TAIR.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..501
FT /note="Endoglucanase 1"
FT /id="PRO_0000249254"
FT ACT_SITE 96
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 422
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 473
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 482
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CONFLICT 330
FT /note="L -> I (in Ref. 1; AAC16418)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 501 AA; 55419 MW; 913F592A058505B7 CRC64;
MALYLSSSRL ITFLSFILLL SNGFSSSSSR PSIHHRHHLD NHNYKDALSK SILFFEGQRS
GKLPPNQRMT WRSNSGLSDG SALNVDLVGG YYDAGDNMKF GFPMAFTTTM LSWSLIEFGG
LMKSELPNAK DAIRWATDFL LKATSHPDTI YVQVGDPNMD HACWERPEDM DTPRSVFKVD
KNNPGSDIAG EIAAALAAAS IVFRKCDPSY SNHLLQRAIT VFTFADKYRG PYSAGLAPEV
CPFYCSYSGY QDELLWGAAW LQKATNNPTY LNYIKANGQI LGADEFDNMF SWDNKHVGAR
ILLSKEFLIQ KVKSLEEYKE HADSFICSVL PGASSSQYTP GGLLFKMGES NMQYVTSTSF
LLLTYAKYLT SARTVAYCGG SVVTPARLRS IAKKQVDYLL GGNPLKMSYM VGYGLKYPRR
IHHRGSSLPS VAVHPTRIQC HDGFSLFTSQ SPNPNDLVGA VVGGPDQNDQ FPDERSDYGR
SEPATYINAP LVGALAYLAR S
