ID   GUN1_FOMML              Reviewed;          27 AA.
AC   C0HLV0;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   03-AUG-2022, entry version 5.
DE   RecName: Full=Endoglucanase gh5 {ECO:0000303|PubMed:33892036};
DE            EC=3.2.1.4 {ECO:0000269|PubMed:33892036};
DE   AltName: Full=Glyoside hydrolase family 5 endoglucanase {ECO:0000303|PubMed:33892036};
DE   Flags: Fragment;
OS   Fomes meliae (Fomitopsis meliae).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Polyporales; Fomitopsis.
OX   NCBI_TaxID=383679 {ECO:0000303|PubMed:33892036};
RN   [1]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, ACTIVITY REGULATION,
RP   CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=33892036; DOI=10.1016/j.ijbiomac.2021.04.110;
RA   Patel A., Shah A.;
RT   "Purification and characterization of novel, thermostable and non-
RT   processive GH5 family endoglucanase from Fomitopsis meliae CFA 2.";
RL   Int. J. Biol. Macromol. 182:1161-1169(2021).
CC   -!- FUNCTION: Endoglucanase (EG) that cleaves the internal beta-1,4-
CC       glucosidic bonds in cellulose. {ECO:0000269|PubMed:33892036}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000269|PubMed:33892036};
CC   -!- ACTIVITY REGULATION: Activity is stimulated by zinc ions, potassium
CC       ions and DTT (PubMed:33892036). Activity is inhibited by manganese and
CC       chloride ions (PubMed:33892036). {ECO:0000269|PubMed:33892036}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=12 mg/ml for carboxymethylcellulose (at pH 4.8 and 55 degrees
CC         Celsius) {ECO:0000269|PubMed:33892036};
CC         Vmax=556.58 umol/min/mg enzyme toward carboxymethylcellulose (at pH
CC         4.8 and 55 degrees Celsius) {ECO:0000269|PubMed:33892036};
CC         Note=Kcat is 129.41/sec for hydrolysis of carboxymethylcellulose (at
CC         pH 4.8 and 55 degrees Celsius). {ECO:0000269|PubMed:33892036};
CC       pH dependence:
CC         Optimum pH is 4.8 (PubMed:33892036). Active from pH 4 to 5
CC         (PubMed:33892036). Activity is significantly reduced at pH 6 and is
CC         not observed at pH 7 (PubMed:33892036).
CC         {ECO:0000269|PubMed:33892036};
CC       Temperature dependence:
CC         Optimum temperature is 70 degrees Celsius (PubMed:33892036).
CC         Thermostable (PubMed:33892036). Highly stable at 50, 60 and 70
CC         degrees Celsius (PubMed:33892036). {ECO:0000269|PubMed:33892036};
CC   -!- MASS SPECTROMETRY: Mass=38870; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:33892036};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; C0HLV0; -.
DR   SMR; C0HLV0; -.
DR   GO; GO:0008810; F:cellulase activity; IDA:UniProtKB.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN           1..27
FT                   /note="Endoglucanase gh5"
FT                   /id="PRO_0000452749"
FT   ACT_SITE        6
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P07982"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:33892036"
FT   NON_TER         27
FT                   /evidence="ECO:0000303|PubMed:33892036"
SQ   SEQUENCE   27 AA;  2911 MW;  A3BEEAA370C44A47 CRC64;
     QAVLSETGGG DTASCDEYLY QELAYVK