GUN1_PERAE
ID GUN1_PERAE Reviewed; 494 AA.
AC P05522;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Endoglucanase 1;
DE EC=3.2.1.4;
DE AltName: Full=Abscission cellulase 1;
DE AltName: Full=Endo-1,4-beta-glucanase 1;
DE Flags: Precursor;
GN Name=CEL1;
OS Persea americana (Avocado).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Persea.
OX NCBI_TaxID=3435;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX AGRICOLA=IND91054589; DOI=10.1007/BF00166456;
RA Tucker M.L., Durbin M.L., Clegg M.T., Lewis L.N.;
RT "Avocado cellulase: nucleotide sequence of a putative full-length cDNA
RT clone and evidence for a small gene family.";
RL Plant Mol. Biol. 9:197-203(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Hass;
RX PubMed=2259345; DOI=10.1007/bf00315799;
RA Cass L.G., Kirven K.A., Christoffersen R.E.;
RT "Isolation and characterization of a cellulase gene family member expressed
RT during avocado fruit ripening.";
RL Mol. Gen. Genet. 223:76-86(1990).
CC -!- FUNCTION: Involved in ripening fruit process.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- DEVELOPMENTAL STAGE: In ripening fruit.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; M17634; AAA32912.1; -; mRNA.
DR EMBL; X59944; CAA42569.1; -; Genomic_DNA.
DR PIR; S11946; S11946.
DR AlphaFoldDB; P05522; -.
DR SMR; P05522; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cellulose degradation; Fruit ripening;
KW Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT SIGNAL 1..25
FT CHAIN 26..494
FT /note="Endoglucanase 1"
FT /id="PRO_0000007955"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 411
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 462
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 471
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 254
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 359
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 494 AA; 54079 MW; 763009AD15575C31 CRC64;
MDCSSPLSLF HLLLVCTVMV KCCSASDLHY SDALEKSILF FEGQRSGKLP TNQRLTWRGD
SGLSDGSSYH VDLVGGYYDA GDNLKFGLPM AFTTTMLAWG IIEFGCLMPE QVENARAALR
WSTDYLLKAS TATSNSLYVQ VGEPNADHRC WERPEDMDTP RNVYKVSTQN PGSDVAAETA
AALAAASIVF GDSDSSYSTK LLHTAVKVFE FADQYRGSYS DSLGSVVCPF YCSYSGYNDE
LLWGASWLHR ASQNASYMTY IQSNGHTLGA DDDDYSFSWD DKRVGTKVLL SKGFLQDRIE
ELQLYKVHTD NYICSLIPGT SSFQAQYTPG GLLYKGSASN LQYVTSTAFL LLTYANYLNS
SGGHASCGTT TVTAKNLISL AKKQVDYILG QNPAKMSYMV GFGERYPQHV HHRGSSLPSV
QVHPNSIPCN AGFQYLYSSP PNPNILVGAI LGGPDNRDSF SDDRNNYQQS EPATYINAPL
VGALAFFAAN PVTE
