ID   GUN1_RUMJO              Reviewed;          25 AA.
AC   P17877;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Endoglucanase 1;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase 1;
DE   AltName: Full=Endo-1,4-beta-glucanase 1;
DE   Flags: Fragment;
OS   Ruminiclostridium josui (Clostridium josui).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminiclostridium.
OX   NCBI_TaxID=1499;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=2500422; DOI=10.1128/jb.171.7.4076-4079.1989;
RA   Fujino T., Sukhumavasi J., Sasaki T., Ohmiya K., Shimizu S.;
RT   "Purification and properties of an endo-1,4-beta-glucanase from Clostridium
RT   josui.";
RL   J. Bacteriol. 171:4076-4079(1989).
CC   -!- FUNCTION: This enzyme hydrolyzes cellotetraose, cellopentaose, and
CC       cellohexaose to cellobiose and cellotriose but does not hydrolyze
CC       cellobiose or cellotriose.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 6.8 with carboxymethyl cellulose (CmC) as substrate.;
CC       Temperature dependence:
CC         Optimum temperature is 60 degrees Celsius with carboxymethyl
CC         cellulose (CmC) as substrate.;
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DR   PIR; A45920; A45920.
DR   AlphaFoldDB; P17877; -.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Polysaccharide degradation.
FT   CHAIN           1..>25
FT                   /note="Endoglucanase 1"
FT                   /id="PRO_0000184077"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..25
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         25
SQ   SEQUENCE   25 AA;  2808 MW;  F68D1B4D3BD9DFCB CRC64;
     YDASLKPNLQ IPQKNIPNND AVNIK