AMT3_ARCFU
ID AMT3_ARCFU Reviewed; 388 AA.
AC O28525;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Ammonium transporter Amt3 {ECO:0000305};
DE AltName: Full=Af-Amt3 {ECO:0000303|PubMed:24958855};
GN Name=amt3 {ECO:0000305};
GN OrderedLocusNames=AF_1749 {ECO:0000312|EMBL:AAB89501.1};
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP FUNCTION.
RX PubMed=24958855; DOI=10.1073/pnas.1406409111;
RA Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.;
RT "Direct observation of electrogenic NH4(+) transport in ammonium transport
RT (Amt) proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9995-10000(2014).
CC -!- FUNCTION: Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3))
CC (PubMed:24958855). Transport is electrogenic (PubMed:24958855).
CC Transport the ammonium and methylammonium cation with high specificity
CC (PubMed:24958855). {ECO:0000269|PubMed:24958855}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:O29285}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O29285};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; AE000782; AAB89501.1; -; Genomic_DNA.
DR PIR; D69468; D69468.
DR RefSeq; WP_010879245.1; NC_000917.1.
DR AlphaFoldDB; O28525; -.
DR SMR; O28525; -.
DR STRING; 224325.AF_1749; -.
DR EnsemblBacteria; AAB89501; AAB89501; AF_1749.
DR GeneID; 1484972; -.
DR KEGG; afu:AF_1749; -.
DR eggNOG; arCOG04397; Archaea.
DR HOGENOM; CLU_000445_33_0_2; -.
DR OMA; MACWNSN; -.
DR OrthoDB; 66605at2157; -.
DR PhylomeDB; O28525; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 3: Inferred from homology;
KW Ammonia transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..388
FT /note="Ammonium transporter Amt3"
FT /id="PRO_0000453007"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT SITE 157
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
FT SITE 305
FT /note="Twin-His motif. Important for optimum substrate
FT conductance"
FT /evidence="ECO:0000250|UniProtKB:P69681"
SQ SEQUENCE 388 AA; 40252 MW; DD59F362FBBD8A8A CRC64;
MQSGDISWLL ISTALVVLMT PALGLFYGGM VRAKNVLSTI TMCFVALAIV SVQWILLGYT
IAFGDDVSGL TGNLVFIGLR NVSMSDYGFV AFQMAFAAIT LAIIVSALAE RVKLSAFLLF
GILWTTFVYD PIAHWVWGGG WLAGIGALDF AGGTVVHISS GFSALALALL VGRRTGYGEY
NIEPHNIPMT LLGTALLWFG WFGFNAGSAL EANDVALNAF LVTNTSAAMG ALSWMFASWL
KGKPGSLGVA SGAIAGLVAI TPAAGYVDPL SALVIGGVAG VVCYAAMLLR VRAGVDESLD
AWAVHGVGGL WGAIATGIFA SVGATGLVAG NAQQLLLQII AALVATAYAF LVTLILAKAV
DAAVGLRVSS QEEYVGLDLS QHEEVAYT
