GUN20_ORYSJ
ID GUN20_ORYSJ Reviewed; 516 AA.
AC Q6ZA06; A0A0P0XFV4; Q0J5Y8;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Endoglucanase 20;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 20;
DE AltName: Full=OsGLU15;
DE Flags: Precursor;
GN Name=GLU15; OrderedLocusNames=Os08g0387400, LOC_Os08g29770;
GN ORFNames=P0434E03.25;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP004689; BAD05437.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23627.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05264.1; -; Genomic_DNA.
DR EMBL; AK071784; BAG92694.1; -; mRNA.
DR RefSeq; XP_015650922.1; XM_015795436.1.
DR AlphaFoldDB; Q6ZA06; -.
DR SMR; Q6ZA06; -.
DR STRING; 4530.OS08T0387400-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q6ZA06; -.
DR PRIDE; Q6ZA06; -.
DR EnsemblPlants; Os08t0387400-01; Os08t0387400-01; Os08g0387400.
DR GeneID; 4345463; -.
DR Gramene; Os08t0387400-01; Os08t0387400-01; Os08g0387400.
DR KEGG; osa:4345463; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q6ZA06; -.
DR OMA; DGNADHN; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6ZA06; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..516
FT /note="Endoglucanase 20"
FT /id="PRO_0000249297"
FT ACT_SITE 93
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 416
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 468
FT /evidence="ECO:0000250"
FT ACT_SITE 477
FT /evidence="ECO:0000250"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 516 AA; 55111 MW; 1DFC890CB93E2872 CRC64;
MAAGMVATMV LLTCLAAGGL VVGAEEDGGG GGLGRLGSPD YGDALAKAIL FFEGQRSGRL
PANQRATWRG DSALTDGREE NVNLTGGYYD AGDNVKFGYP MAFTVTLLGW SAVEYGAAVA
AAGELGNLRA AIRWGADFLL RAHASPTTLY TQVGDGNADH QCWERPEDMD TPRTLYKITA
DSPGSEAAAE ASAALAAAYV ALKDDGDTAF SSRLLAASRS LFDFANNYRG SFQSSCPFYC
SYSGFQDELL WASAWLFKAT RDAKYLDFLT NNQGSSNPVN EFSWDNKYAG AQMLAAQEYL
GGRTQLARYK DNLDSFVCAL MPNSGNVQIR TTPGGLLFTR DSVNLQYTTT ATLVLSIYSK
VLKSSGSRGV RCSAATFSPN QISSFATSQV DYILGKNPLG MSYMVGFSTK FPRRIHHRGS
SIPSIKVLSR KVTCKEGFSS WLPTSDPNPN IHVGAIVGGP DGNDQFSDNR GDSSHSEPAT
YINAAFVGAC AAAMGQKQVV KLEEPADNLE SMVSTY