位置:首页 > 蛋白库 > GUN20_ORYSJ
GUN20_ORYSJ
ID   GUN20_ORYSJ             Reviewed;         516 AA.
AC   Q6ZA06; A0A0P0XFV4; Q0J5Y8;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Endoglucanase 20;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 20;
DE   AltName: Full=OsGLU15;
DE   Flags: Precursor;
GN   Name=GLU15; OrderedLocusNames=Os08g0387400, LOC_Os08g29770;
GN   ORFNames=P0434E03.25;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP004689; BAD05437.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF23627.1; -; Genomic_DNA.
DR   EMBL; AP014964; BAT05264.1; -; Genomic_DNA.
DR   EMBL; AK071784; BAG92694.1; -; mRNA.
DR   RefSeq; XP_015650922.1; XM_015795436.1.
DR   AlphaFoldDB; Q6ZA06; -.
DR   SMR; Q6ZA06; -.
DR   STRING; 4530.OS08T0387400-01; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; Q6ZA06; -.
DR   PRIDE; Q6ZA06; -.
DR   EnsemblPlants; Os08t0387400-01; Os08t0387400-01; Os08g0387400.
DR   GeneID; 4345463; -.
DR   Gramene; Os08t0387400-01; Os08t0387400-01; Os08g0387400.
DR   KEGG; osa:4345463; -.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; Q6ZA06; -.
DR   OMA; DGNADHN; -.
DR   OrthoDB; 1195424at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   Genevisible; Q6ZA06; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..516
FT                   /note="Endoglucanase 20"
FT                   /id="PRO_0000249297"
FT   ACT_SITE        93
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        416
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        468
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        477
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        83
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   516 AA;  55111 MW;  1DFC890CB93E2872 CRC64;
     MAAGMVATMV LLTCLAAGGL VVGAEEDGGG GGLGRLGSPD YGDALAKAIL FFEGQRSGRL
     PANQRATWRG DSALTDGREE NVNLTGGYYD AGDNVKFGYP MAFTVTLLGW SAVEYGAAVA
     AAGELGNLRA AIRWGADFLL RAHASPTTLY TQVGDGNADH QCWERPEDMD TPRTLYKITA
     DSPGSEAAAE ASAALAAAYV ALKDDGDTAF SSRLLAASRS LFDFANNYRG SFQSSCPFYC
     SYSGFQDELL WASAWLFKAT RDAKYLDFLT NNQGSSNPVN EFSWDNKYAG AQMLAAQEYL
     GGRTQLARYK DNLDSFVCAL MPNSGNVQIR TTPGGLLFTR DSVNLQYTTT ATLVLSIYSK
     VLKSSGSRGV RCSAATFSPN QISSFATSQV DYILGKNPLG MSYMVGFSTK FPRRIHHRGS
     SIPSIKVLSR KVTCKEGFSS WLPTSDPNPN IHVGAIVGGP DGNDQFSDNR GDSSHSEPAT
     YINAAFVGAC AAAMGQKQVV KLEEPADNLE SMVSTY
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024