ID   GUN22_ORYSJ             Reviewed;         556 AA.
AC   Q6H3Z9; Q0J1Y7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Endoglucanase 22;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 22;
DE   AltName: Full=OsGLU11;
DE   Flags: Precursor;
GN   Name=GLU11; OrderedLocusNames=Os09g0394300, LOC_Os09g23084;
GN   ORFNames=B1339H09.3, OSJNOa018M17.6;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-556.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD26493.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD26550.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF25006.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP006453; BAD26493.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP007149; BAD26550.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008215; BAF25006.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK105580; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015612486.1; XM_015757000.1.
DR   AlphaFoldDB; Q6H3Z9; -.
DR   SMR; Q6H3Z9; -.
DR   STRING; 4530.OS09T0394300-01; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; Q6H3Z9; -.
DR   PRIDE; Q6H3Z9; -.
DR   GeneID; 4346968; -.
DR   KEGG; osa:4346968; -.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; Q6H3Z9; -.
DR   OrthoDB; 1195424at2759; -.
DR   Proteomes; UP000000763; Chromosome 9.
DR   Proteomes; UP000059680; Chromosome 9.
DR   Genevisible; Q6H3Z9; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..556
FT                   /note="Endoglucanase 22"
FT                   /id="PRO_0000249299"
FT   ACT_SITE        108
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        450
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        502
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        511
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
SQ   SEQUENCE   556 AA;  61243 MW;  B10106C3E6E635ED CRC64;
     MSRGRARLQP PPPGTRTTTL AAVLVLVLLA VVALPLRCDA ASAGGEEEEE QQPLDYREAL
     EKSLLYFEAQ RSGRLPYSQR VTWRGHSGLT DGLQQGVDLV GGYYDAGDHV KFGLPMAFTV
     TMLSWGAIDF AADIAAAGEW RHALEAIKWG TDYFVKAHTH PFVYWAEVGD GDTDHYCWQR
     PEDMTTSRQA YRVDRDNPGS DLAGETAAAL AAASIVFRRS DPHYSHLLLH HAQQLFEFGD
     TYRGSYDSSI EEVRSYYASV SGYHDELLWA ALWLHRATGK EEYLRYAVDN ADSFGGVGWA
     ITEFSWDVKY AGLQVLAAKL LLDGDPQAAA HRGVLEKYRE KAEHYLCACL GRNINGADNV
     DRSPGGMLYV RQWNNLQYAS SAAFLLTAYS HYLSSSSASA SAALRCPGGA AAAAEMVSLA
     RSQADYILGR NPLRLSYMVG YGRRYPARVH HRGASIVSHK EDGRFIGCVQ GFDDWFGRGR
     ANPNVLAGAI VGGPSRRDEF RDDRANYMQT EACTYNTAPM VAVFARLHRL TTAITTAAAA
     EDPDGGSPDR RSVDRR