GUN23_ORYSJ
ID GUN23_ORYSJ Reviewed; 515 AA.
AC Q69NF5; A0A0P0XPN9; Q0J054;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Endoglucanase 23;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 23;
DE AltName: Full=OsGLU12;
DE Flags: Precursor;
GN Name=GLU12; OrderedLocusNames=Os09g0530200, LOC_Os09g36060;
GN ORFNames=OJ1531_B07.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR EMBL; AP005682; BAD33772.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25661.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT09088.1; -; Genomic_DNA.
DR EMBL; AK106815; BAG97842.1; -; mRNA.
DR RefSeq; XP_015651341.1; XM_015795855.1.
DR AlphaFoldDB; Q69NF5; -.
DR SMR; Q69NF5; -.
DR STRING; 4530.OS09T0530200-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q69NF5; -.
DR PRIDE; Q69NF5; -.
DR EnsemblPlants; Os09t0530200-01; Os09t0530200-01; Os09g0530200.
DR GeneID; 4347660; -.
DR Gramene; Os09t0530200-01; Os09t0530200-01; Os09g0530200.
DR KEGG; osa:4347660; -.
DR eggNOG; ENOG502QV58; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q69NF5; -.
DR OMA; TINCGNI; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; Q69NF5; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0010047; P:fruit dehiscence; IEA:EnsemblPlants.
DR GO; GO:1990059; P:fruit valve development; IEA:EnsemblPlants.
DR GO; GO:0009828; P:plant-type cell wall loosening; IEA:EnsemblPlants.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..515
FT /note="Endoglucanase 23"
FT /id="PRO_0000249300"
FT ACT_SITE 95
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 426
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 477
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 486
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 384
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 452
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 515 AA; 55453 MW; A694BD5C4401C259 CRC64;
MALLSAPVRR RRSRVRVLLV CCCLLLALAA PSAAAAAAGH DYGDALAKSI LFFEGQRSGR
LPAAGQRAAW RGDSAVSDGG AAGVDLEGGY YDAGDNVKFG FPMAFTATML AWGVVEFGDA
MPPAERAHAA DAVRWATDYL LKTISHPGVI FIQVGDPTKD HGCWERPEDM DTARTVYNIS
AARPGSDVAG ETAAALAAAS MVFRDDDPAY AARLLAGARS AFEFADEHKG AYSDDPELRA
GGCPFYCDFD GYQDELLWGA AWLRRASKEG TYLDYIQNNG KTLGAEDSTN EFGWDNKHAG
INVLVSKEFI DGEVLSLQSY KEFADGFICT LIPESSSPHI TYTPGGMIYK PGGSNMQHVT
SISFLLLTYA KYLSNSSRTV NCGNVSVGPA TLQQLARKQA DYILGDNPMK MSYMVGYGDR
YPQRIHHRGS SLPSIKSHPQ RIACNDGTPY YNSSSPNPNP LIGAVVGGPG EDDVYEDDRA
DFRKSEPTTY INAPLVGVLA YLVGNPDPGQ GHVRH
