GUN24_ARATH
ID GUN24_ARATH Reviewed; 497 AA.
AC Q93YQ7; Q9SVJ2;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Endoglucanase 24;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 24;
DE Flags: Precursor;
GN OrderedLocusNames=At4g39010; ORFNames=F19H22.110;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA Libertini E., Li Y., McQueen-Mason S.J.;
RT "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL J. Mol. Evol. 58:506-515(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB38821.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB80564.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL035679; CAB38821.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL161594; CAB80564.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002687; AEE87007.1; -; Genomic_DNA.
DR EMBL; AY059825; AAL24307.1; -; mRNA.
DR EMBL; BT002204; AAN72215.1; -; mRNA.
DR PIR; T06061; T06061.
DR RefSeq; NP_568050.1; NM_120061.2.
DR AlphaFoldDB; Q93YQ7; -.
DR SMR; Q93YQ7; -.
DR STRING; 3702.AT4G39010.1; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q93YQ7; -.
DR PRIDE; Q93YQ7; -.
DR ProteomicsDB; 247245; -.
DR EnsemblPlants; AT4G39010.1; AT4G39010.1; AT4G39010.
DR GeneID; 830056; -.
DR Gramene; AT4G39010.1; AT4G39010.1; AT4G39010.
DR KEGG; ath:AT4G39010; -.
DR Araport; AT4G39010; -.
DR TAIR; locus:2120242; AT4G39010.
DR eggNOG; ENOG502QV58; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; Q93YQ7; -.
DR OMA; TINCGNI; -.
DR OrthoDB; 1195424at2759; -.
DR PhylomeDB; Q93YQ7; -.
DR BioCyc; ARA:AT4G39010-MON; -.
DR PRO; PR:Q93YQ7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93YQ7; baseline and differential.
DR Genevisible; Q93YQ7; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0010047; P:fruit dehiscence; IGI:TAIR.
DR GO; GO:1990059; P:fruit valve development; IGI:TAIR.
DR GO; GO:0009828; P:plant-type cell wall loosening; IGI:TAIR.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..497
FT /note="Endoglucanase 24"
FT /id="PRO_0000249276"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 414
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT ACT_SITE 474
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT CARBOHYD 440
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 497 AA; 55495 MW; 1F3EC2493D36BDDD CRC64;
MKLHLHPLSL VFFFFFFFFR PTMSSNQHDY SDALSKSILF FEGQRSGYLP NDQRMTWRRN
SGLSDGWTHN IDLTGGYYDA GDNVKFNFPM AFTTTMLAWS VIEFGEFMPS SELRNSLVAL
RWSSNYLLKS VSQLPNRIFV QVGDPIADHN CWERPEDMDT PRTAYAVNAP NPASEVAGET
TAALSAASIA FRSSDPGYSQ TLLQNAVKTF QFADMYRGAY SSNDDIKNDV CPFYCDFNGF
QDELLWGAAW LRKATGDESY LNYIESNREP FGANDNVDEF GWDNKVGGLN VLVSKEVIEG
NMYNLEAYKA SAESFMCSLV PESSGPHVEY TSAGLLYKPG GSQLQHATTI SFLLLVYAQY
LSRSSLSLNC GTLTVPPDYL RRLAKKQVDY ILGNNPMGLS YMVGYGERYP KRIHHRGSSL
PSIVDHPEAI RCKDGSVYFN STEPNPNVLI GAVVGGPGED DMYDDDRSDF RKSEPTTYIN
APFVGVLAYF AANPGSS
