GUN24_ORYSJ
ID GUN24_ORYSJ Reviewed; 528 AA.
AC Q69SG5; A0A0P0XQY5; Q0J030;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Endoglucanase 24;
DE EC=3.2.1.4;
DE AltName: Full=Endo-1,4-beta glucanase 24;
DE Flags: Precursor;
GN OrderedLocusNames=Os09g0533900, LOC_Os09g36350; ORFNames=OJ1112_E07.17-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP005092; BAD33331.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25685.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT09128.1; -; Genomic_DNA.
DR EMBL; AK100449; BAG94612.1; -; mRNA.
DR RefSeq; XP_015612534.1; XM_015757048.1.
DR AlphaFoldDB; Q69SG5; -.
DR SMR; Q69SG5; -.
DR STRING; 4530.OS09T0533900-01; -.
DR CAZy; GH9; Glycoside Hydrolase Family 9.
DR PaxDb; Q69SG5; -.
DR PRIDE; Q69SG5; -.
DR EnsemblPlants; Os09t0533900-01; Os09t0533900-01; Os09g0533900.
DR EnsemblPlants; Os09t0533900-02; Os09t0533900-02; Os09g0533900.
DR GeneID; 4347685; -.
DR Gramene; Os09t0533900-01; Os09t0533900-01; Os09g0533900.
DR Gramene; Os09t0533900-02; Os09t0533900-02; Os09g0533900.
DR KEGG; osa:4347685; -.
DR eggNOG; ENOG502QQZQ; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; Q69SG5; -.
DR OMA; YLINCHP; -.
DR OrthoDB; 1195424at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR ExpressionAtlas; Q69SG5; baseline and differential.
DR Genevisible; Q69SG5; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR PROSITE; PS60032; GH9_1; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..528
FT /note="Endoglucanase 24"
FT /id="PRO_0000249301"
FT ACT_SITE 109
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT ACT_SITE 446
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT ACT_SITE 492
FT /evidence="ECO:0000250"
FT ACT_SITE 501
FT /evidence="ECO:0000250"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 487
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 528 AA; 56950 MW; 1BB3653AD5047075 CRC64;
MGSKTKGCCG WLIVALVASL VATAAVVAIM KKKVGGGSGR KLKPLPVPGP PGAIDSKYGD
ALGVALQFFQ VQKAGKLENN QIPWRGDSAL DDGKPAGLDL SKGMYDAGDH IKFSFPMAFT
ATVLSWSILE YGDQMSATKQ LDPALDALRW ITDFLVNAHP SDNVFYIQVG DPDLDHNCWE
RPETMSEKRP LTQINTKSPG SDVAAEAAAA MASASIVFKS RDTTYSDSLL QHAQKLFTFA
DTYKGLASDT YPKLQNYYNS TGYQDELLWA ASWLYHATGD QTYLSYVTVE NGKAFADWGR
PTWFSWDDKL AGTQVLLSRL NFFGSKQTSN AENMGLKMYR DTAEAVICGL LPDSPSATAS
RTGGGLVWIS GWNSLQHATN AAFLAVVYSD YMLTSQTAAV QCSGKYYSPT DIRNFAISQA
NYILGDNPMK LSYLVGYGSS YPQQVHHRGA SIPADAKTGC KGFQYLHSTS PNPNVAMGAL
VGGPFQNDTF VDSRDNAVQT ESSTYNSGTL VGLLSGLVTT SSVAQSFT
